血清miR-18a、miR-485-5p对HPV阳性宫颈癌的诊断价值

  目的  探讨宫颈癌患者伴人乳头瘤病毒(HPV)与单纯宫颈癌患者血清微小RNA(miR)-18a，miR-485-5p表达差异，分析二指标对HPV阳性宫颈癌的诊断价值。  方法  选取2020年1月—2021年1月黄河三门峡医院收治的91例HPV阳性宫颈癌患者为HPV阳性宫颈癌组，62例HPV阳性非宫颈癌妇女为HPV阳性组，57名体检健康妇女为对照组，采用qRT-PCR检测血清miR-18a、miR-485-5p的表达。采用受试者操作特征曲线(ROC)分析血清miR-18a、miR-485-5p对HPV阳性宫颈癌的诊断价值。  结果  HPV阳性宫颈癌组血清miR-18a表达高于对照组和HPV阳性组，而miR-485-5p表达低于对照组和HPV阳性组(均P＜0.05)。血清miR-18a、miR-485-5p表达与HPV阳性宫颈癌患者FIGO分期、间质浸润深度、是否淋巴结转移有关(均P＜0.05)。ROC曲线显示，miR-18a、miR-485-5p、联合诊断HPV阳性宫颈癌的AUC分别为0.752、0.802、0.876，灵敏度分别为67.03%、75.82%、83.52%，特异度分别为77.42%、74.19%、83.87%。联合诊断HPV阳性宫颈癌的AUC大于miR-18a、miR-485-5p单独诊断(均P＜0.05)。  结论  HPV阳性宫颈癌血清miR-18a高表达，miR-485-5p低表达，可作为HPV阳性宫颈癌辅助诊断指标。 

Diagnostic value of serum miR-18a and miR-485-5p for HPV-positive cervical cancer

  Objective  To explore the difference of serum MicroRNA (miR)-18a and miR-485-5p expression between cervical cancer patients with human papillomavirus (HPV) and simple cervical cancer patients, and analyze the diagnostic value of the two indicators for HPV-positive cervical cancer.  Methods  A total of 91 patients with HPV-positive cervical cancer admitted to Yellow River Sanmenxia Hospital from January 2020 to January 2021 were selected as the HPV-positive cervical cancer group. Sixty-two women with HPV-positive non-cervical cancer were placed in the HPV-positive group, and 57 healthy women based on physical examination were placed in the control group. Serum miR-18a and miR-485-5p expression was detected by qRT-PCR. Receiver operating characteristic (ROC) curve was used to analyse the diagnostic value of two indicators for HPV-positive cervical cancer.  Results  The expression of serum miR-18a in HPV-positive cervical cancer group was higher, while the expression of miR-485-5p was lower than those in the control group and HPV-positive cervical cancer group (P < 0.05). Serum miR-18a and miR-485-5p expression was associated with FIGO stage, depth of interstitial infiltration and lymph node metastasis in patients with HPV-positive cervical cancer (P < 0.05). The ROC curves showed that the area under the curve (AUC) for miR-18a and miR-485-5p and their combination for the diagnosis of HPV-positive cervical cancer were 0.752, 0.802 and 0.876, respectively, with sensitivities of 67.03%, 75.82% and 83.52%, and specificities of 77.42%, 74.19% and 83.87%, respectively. The AUC of the combined diagnosis of HPV-positive cervical cancer was greater than that of miR-18a and miR-485-5p alone (P < 0.05).  Conclusion  High expression of serum miR-18a and low expression of miR-485-5p in HPV-positive cervical cancer can be used as an auxiliary diagnostic indicator for HPV-positive cervical cancer.