红肉火龙果果原汁对肾性高血压大鼠的降压作用研究

目的 研究红肉火龙果果原汁(RPJ)对肾性高血压大鼠(RHR)的降压作用.方法 采用两肾一夹(2K1C)针灸针缩窄法制备大鼠肾性高血压模型,将造模成功的大鼠随机分为假手术组、模型组、卡托普利组和RPJ高、中、低剂量组,连续灌胃8周末,测定各组大鼠收缩压(SBP),记录左肾重量与右肾重量比值(LK/RK)和左心室重量指数(LVMI);光学显微镜观察心肌组织及主动脉弓病理学变化;ELISA法测定大鼠血浆、心肌及肾脏血管紧张素Ⅱ(AngⅡ)、醛固酮(Ald)及内皮素-1(ET-1)含量.结果 与模型组相比,RPJ高、中剂量组大鼠血压均明显降低(P<0.05),LK/RK比值明显升高(P<0.05),其中高剂量组能够明显降低LVMI值(P<0.05);RPJ高、中剂量组可以使血浆、心肌及肾脏中的AngⅡ、Ald和ET-1含量不同程度减少(P<0.01或P<0.05),RPJ低剂量组血浆AngⅡ含量明显下降(P<0.05).结论 RPJ对肾性高血压有较好的治疗作用,值得进一步深入研究.

Study on the antihypertensive effect of red pitaya juice in renal hypertensive rats

Objective To explore the antihypertensive effects of red pitaya juice ( RPJ) in renal hypertensive rats ( RHR) . Methods The model of renal hypertension rats was established by two-kidney one-clip (2K1C) acupuncture needle narrowed method. After opera-tion,the rats were randomly assigned into 6 groups:sham-operated group,model group,captopril group,high-dose,middle-dose,low-dose groups of RPJ. Then drug was given continuously by ig for 8 weeks. After 8-week treatment,the systemic blood pressure ( SBP) was measured;The ratio of left kidney/right kidney ( LK/RK ) and the left ventricular mass index ( LVMI ) were recorded;pathological changes of myocardial tissue and aortic arch were observed by optical microscope;and angiotensinⅡ( AngⅡ) ,aldosterone ( Ald) and endothelin-1 (ET-1) in plasma,heart and kidney tissue were measured by ELISA. Results Compared with model group,the SBP of RHR in RPJ high-dose,middle-dose groups were significantly reduced (P<0. 05),while the levels of LK/RK were increased (P<0. 05);the LVMI in RPJ high-dose were significantly lower (P<0. 05);AngⅡ,Ald and ET-1 level in plasma,heart and kidney tissue of RPJ high-dose and middle-dose groups were reduced to varying degrees (P<0. 01 or P<0. 05);AngⅡ in plasma of RPJ low-dose was significantly reduced (P<0. 05). Conclusions RPJ has a better effect on the treatment of renal hypertension,which is worth fur-ther research.