Differential regulation of leucine-rich primary response gene 1 (LRPR1) mRNA expression in rat testis and ovary |
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Authors: | Slegtenhorst-Eegdeman KE; Verhoef-Post M; Parvinen M; Grootegoed JA; Themmen AP |
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Institution: | Department of Endocrinology and Reproduction, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The Netherlands. |
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Abstract: | In immature rat Sertoli cells, leucine-rich primary response gene 1 (LRPR1)
represents a follicle stimulating hormone (FSH)-responsive gene; the
function of the encoded protein is not yet known. LRPR1 mRNA expression is
up-regulated very rapidly and specifically by FSH, both in cultured Sertoli
cells and in vivo in regulation in more detail, in testis and ovary of
fetal, immature, and adult rats. In addition, we have studied the
expression of FSH receptor (FSHR) mRNA in relation to LRPR1 mRNA
expression. In rat testis, LRPR1 mRNA and FSHR mRNA followed a similar
expression pattern, during postnatal development and also at different
stages of the spermatogenic cycle in the adult rat. Furthermore, after
short-term challenge of the FSH signal transduction pathway in intact
immature rats by injection with a relatively high dose of FSH, an inverse
relationship between LRPR1 mRNA (up-regulation) and FSHR mRNA expression
(down-regulation) was observed. Similar studies in the ovary provided
completely different results. LRPR1 mRNA in the postnatal ovary is present
well before expression of FSHR mRNA can be first detected. In addition,
incubation of ovaries of immature rats with FSH or dibutyryl cyclic AMP
(dbcAMP) did not result in up- regulation of LRPR1 mRNA expression. During
fetal development, the LRPR1 mRNA expression pattern involved many more
tissues, in contrast to the relatively tissue-specific expression of LRPR1
mRNA in gonads of 21 day old and adult rats. Moreover, LRPR1 mRNA
expression could be detected as early as 12.5 days post-coitum, whereas
FSHR mRNA is absent at this stage of fetal development. We concluded that
the pronounced regulation of LRPR1 by FSH observed in the immature rat
testis does not occur in the ovary. Furthermore, in the ovary LRPR1 mRNA
expression does not appear to be dependent on FSH action. Finally, the
LRPR1 gene product may play a general role during fetal development.
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