三维培养模型中胰岛素样生长因子-I对人牙周膜细胞增殖及碱性磷酸酶活性的影响

目的 了解胰岛素样生长因子-I(IGF-I)在三维培养条件下对人牙周膜细胞(hPDLCs)增殖及碱性磷酸酶(ALP)活性的影响.方法 通过组织块法分离培养hPDLCs.采用旋转细胞培养系统(RCCS)建立三维培养环境.将细胞分为4组,分别为阴性对照组、阳性对照组(三维培养组、IGF-I组)、实验组(三维培养加IGF-I...

The effect of insulin-like growth factor-I on the proliferation and alkaline phosphatase activity of human periodontal ligament cells under three-dimensional culture system

Objective To investigate the effect of insulin-like growth factor-Ⅰ（IGF-Ⅰ） on the proliferation and alkaline phosphatase（ALP） activity of human periodontal ligament cells（hPDLCs） under three-dimensional（3D） culture system. Methods The hPDLCs were cultured from periodontium of human teeth by the outgrowth method. Rotary cell culture system（RCCS） was enrolled to set 3D culture system. Samples were set to four groups: Negative control group, positive control group（3D group, IGF-Ⅰ group）, and experimental group（3D with IGF-Ⅰ group）. Proliferation was tested with methylthiazolyl tetrazolium（MTT）, and ALP activity was assayed by spectrophotometer at 1, 3, 5, 7 d respectively. Results Compared with that of negative control group, cell proliferation increased significantly in 3D with IGF-Ⅰ group since 3 d（P<0.05）. Besides, the cell proliferation of 3D with IGF-Ⅰ group was significantly higher than that of 3D group（P<0.05）. ALP activity of 3D with IGF-Ⅰ group was significantly higher than that of negative control group,and 3D group at 3, 5, 7 d（P<0.05）. Conclusion IGF-Ⅰsignificantly promotes the proliferation and ALP activity of hPDLCs under 3D culture system.