| 侵袭性牙周炎与慢性牙周炎病变位点中人巨细胞病毒的定量检测 |
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| 引用本文： | Li X,Sun QF,Sun YD,Ge SH,Yang PS. 侵袭性牙周炎与慢性牙周炎病变位点中人巨细胞病毒的定量检测[J]. 华西口腔医学杂志, 2011, 29(3): 242-245. DOI: 10.3969/j.issn.1000-1182.2011.03.005 |
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| 作者姓名： | Li X Sun QF Sun YD Ge SH Yang PS |
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| 作者单位： | 1.山东大学口腔医院牙周科, 山东省口腔生物医学重点实验室;2.山东大学医学院医学微生物学教研室, 济南250012 |
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| 摘 要： | 目的 应用实时荧光定量PCR方法 检测侵袭性牙周炎(AgP)及慢性牙周炎(CP)患者龈下样本中人巨细胞病毒(HCMV)的DNA载量,探讨HCMV感染与牙周炎之间的关系.方法 选择18例AgP患者、24例CP患者及15例牙周健康对照者,收集龈下样本114例.构建含有HCMV高保守片段的重组质粒,制备标准品DNA模板,建立...
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| 关 键 词： | 人巨细胞病毒 侵袭性牙周炎 慢性牙周炎 |
| 收稿时间： | 2011-06-25 |
| 修稿时间： | 2011-06-25 |
Quantitative detection of human cytomegalovirus in aggressive and chronic periodontitis lesions |
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| Li Xiao,Sun Qin-Feng,Sun Yun-Dong,Ge Shao-Hua,Yang Pi-Shan. Quantitative detection of human cytomegalovirus in aggressive and chronic periodontitis lesions[J]. West China journal of stomatology, 2011, 29(3): 242-245. DOI: 10.3969/j.issn.1000-1182.2011.03.005 |
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| Authors: | Li Xiao Sun Qin-Feng Sun Yun-Dong Ge Shao-Hua Yang Pi-Shan |
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| Affiliation: | 1. Dept. of Periodontics, School of Stomatology, Shandong University; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China; 2. Dept. of Microbiology, School of Medicine, Shandong University, Jinan 250012, China |
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| Abstract: | Objective To establish a quantitative real-time PCR assay for the detection of human cytomegalovirus(HCMV) DNA load in subgingival specimens from the patients with aggressive and chronic periodontitis,and to investigate the relationship between HCMV infection and the periodontal status.Methods A total of 114 subgingival plaque specimens were taken from 18 subjects with aggressive priodontiti(AgP),24 subjects with chronic periodontitis(CP) and 15 healthy control subjects.Standard quantification was performed... |
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| Keywords: | human cytomegalovirus aggressive periodontitis chronic periodontitis |
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