| As2O3诱导Raji细胞凋亡的基因分析 |
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| 引用本文： | ZHANGZidong LIWeiyu 等. As2O3诱导Raji细胞凋亡的基因分析[J]. 中德临床肿瘤学杂志, 2002, 1(3): 149-151. DOI: 10.1007/BF02851710 |
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| 作者姓名： | ZHANGZidong LIWeiyu 等 |
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| 作者单位： | InstituteofHematology,MedicalCollegeofJinanUniversity,Guangzhou510632,China |
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| 摘 要： | Objective The effect of arsenic trioxide on apoptosis gene expression of Raji cell was explored when Raji cells were incubated with 0.5μmol/L of arsenic trioxide for 6h。Methods Cell culture,extraction and isolation of mRNA,preparation of probes labeled with fluorescence,hybridization technique of DNA chip(each chip containing 200 apoptosis genes,Chinese Shanghai Biostar,In.)were used.Results Arsenic trioxide induced significant changes in 10%(20/200 genes)of the apoptosis genes:18 genes were downregulated,only two upregulated.In particular,inhibitors of apoptosis protein,such as X-linked inhibitor of apoptosis protein,were significantly downregulated.P53 and the other apoptosis genes were also downregulatec.Of the upregulated genes,high expression of heat-shock protein could promote apoptosis of Raji cells.Conclusion The inhibitors of apoptosis protein play an important role in the process of arsenic trioxide-induced apoptosis of Raji cells.
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| 关 键 词： | 淋巴瘤 三氧化二砷 诱导 肿瘤细胞凋亡 基因分析 |
| 收稿时间： | 2002-01-14 |
Gene analysis of arsenic trioxide-induced apoptosis of lymphoma cells |
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| Zidong Zhang,Weiyu Li,Lin Nie,Yuan Zhang. Gene analysis of arsenic trioxide-induced apoptosis of lymphoma cells[J]. The Chinese-German Journal of Clinical Oncology, 2002, 1(3): 149-151. DOI: 10.1007/BF02851710 |
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| Authors: | Zidong Zhang Weiyu Li Lin Nie Yuan Zhang |
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| Affiliation: | (1) Institute of Hematology, Medical College of Jinan University, 510632 Guangzhou, China |
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| Abstract: | Objective The effect of arsenic trioxide on apoptosis gene expression of Raji cell was explored when Raji cells were incubated with 0.5 μmol/L of arsenic trioxide for 6 h. Methods Cell culture, extraction and isolation of mRNA, preparation of probes labeled with fluorescence, hybridization technique of DNA chip (each chip containing 200 apoptosis genes, Chinese Shanghai Biostar In.) were used. Results Arsenic trioxide induced significant changes in 10% (20/200 genes) of the apoptosis genes: 18 genes were downregulated, only two upregulated. In particular, inhibitors of apoptosis protein, such as X-linked inhibitor of apoptosis protein, were significantly downregulated. P53 and the other apoptosis genes were also downregulated. Of the upregulated genes, high expression of heat-shock protein could promote apoptosis of Raji cells. Conclusion The inhibitors of apoptosis protein play an important role in the process of arsenic trioxide-induced apoptosis of Raji cells. This project was supported by a grant from foundation of Science and Technology of Guangzhou city (2001-Z-037-01) and Guangdong Province (99M1204G). |
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| Keywords: | arsenic trioxide Raji lymphoma cells DNA chip gene expression |
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