低强度He-Ne激光对软骨细胞增殖的影响

目的研究低强度HeNe激光对兔软骨细胞增殖和变异的影响。方法选取3周龄新西兰白兔分离培养软骨细胞,分别在浓度为10%,5%,2.5%的新生牛血清(newborncalfserum,NCS)及无血清4种培养媒介中培养。采用波长为632.8nm,功率为6.5mW的HeNe激光照射软骨细胞,每天分别照射2,8,16,30和45min,共6d。在培养至第13天时,用XTT法检测细胞的活性,了解细胞的增殖情况;用吖啶橙标记软骨细胞DNA,激光共聚焦显微镜下观察软骨细胞形态及DNA的表达。结果(1)XTT结果显示,在营养缺乏培养状态中(5%,2.5%NCS),照射时间为16,30和45min的照射组细胞数量明显增加,与无激光照射组的差异有统计学意义(P<0.01),其中最佳照射时间为30min,实际最佳照射能量密度为9.42J/cm2。(2)照射组软骨细胞形态与正常软骨细胞差异无统计学意义;DNA荧光信号较对照组强;未见显著的形态改变。结论低强度HeNe激光能促进兔软骨细胞的生长,使DNA表达明显增强。

The effect of low intensity He-Ne laser irradiation on chondrocytes proliferation

Objective To investigate the potential effect of low intensity He-Ne laser irradiation on the proli-feration and mutation of rabbit cartilage cells in vitro. Methods The sample of cartilage of New Zealand white rabbits aged 3 weeks were used. The chondrocytes were isolated and suspended in medium with different concentrations (0%, 2.5%, 5% and 10%)of newborn calf serum (NCS). The chondrocytes were treated by He-Ne laser irradiation with the wavelength of 632.8nm, 6.5mW for 2, 8, 16, 30 or 45 minutes daily, respectively, for 6 days. After treatment the chondrocytes were incubated till the 13th day, and the proliferation and activity were assessed by a XTT tests, and DNA expression by acridine orange stain and confocal laser scanning microscope. Results The XTT tests showed that, in nutrition-deficit condition of culture, laser irradiation for 16, 30 and 45 minutes significantly increased the number of the chondrocytes as compared with that without laser irradiation (P<~0.01 ). The optimal irradiation time for proliferation was 30 minutes, and the optimal energy density was 9.42J/cm2. The chondrocytes DNA fluorescence signal was significantly stronger in the irradiation groups than that in the non-irradiation group. However, there was no significant morphological difference between the irradiated and normal chondrocytes. Conclusion Low intensity of He-Ne laser irradiation can promote the proliferation and DNA expression of chondrocytes.