| 高效液相色谱-串联质谱法测定中药材中赭曲霉素A |
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| 引用本文： | 邓鸣,李浩,朱斌. 高效液相色谱-串联质谱法测定中药材中赭曲霉素A[J]. 中国药师, 2014, 0(10): 1652-1655 |
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| 作者姓名： | 邓鸣 李浩 朱斌 |
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| 作者单位： | 广西食品药品检验所 南宁 530021;广西食品药品检验所 南宁 530021;广西食品药品检验所 南宁 530021 |
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| 基金项目： | 科技部重大新药创制项目（编号：2009ZX09308-006）；广西民族医药创新研究与产业化技术平台建设项目（编号：桂科能10124008-15） |
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| 摘 要： | 目的：建立中药材中赭曲霉素A（ OTA）的高效液相色谱-串联质谱（ HPLC-MS/MS）检测方法。方法：样品采用80%甲醇溶液提取,经免疫亲和柱净化,以甲醇-乙腈-0.01 mol·L^-1醋酸铵溶液作为流动相,C18色谱柱分离,电喷雾多反应监测模式进行检测。结果：OTA检测限为0.1μg·kg^-1,平均回收率84.8%~91.2%,相对标准偏差3.6%~8.1%（n=9）。结论：该方法准确、灵敏、简便,适用于中药材中赭曲霉素A的测定。
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| 关 键 词： | 中药材 赭曲霉素A 高效液相色谱-串联质谱法 免疫亲和柱 |
| 收稿时间： | 2014-05-19 |
| 修稿时间： | 2014-07-07 |
Determination of Ochratoxin A in Chinese Herbs by HPLC-MS/MS |
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| Deng Ming,Li Hao and Zhu Bin. Determination of Ochratoxin A in Chinese Herbs by HPLC-MS/MS[J]. China Pharmacist, 2014, 0(10): 1652-1655 |
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| Authors: | Deng Ming Li Hao Zhu Bin |
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| Affiliation: | (Guangxi Institute for Food and Drug Control, Nanning 530021, China) |
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| Abstract: | Objective：To determine ochratoxin A （ OTA ） in Chinese herbs by high performance liquid chromatography-tandem mass spectrometry method （ HPLC-MS/MS） . Methods： The samples were extracted by 80% methanol water solution and purified by immunoaffinity column. The chromatographic separation was carried out on a C18 column, the mobile phase was methanol-acetonitrile-0. 01 mol·L^-1 ammonium acetate, and then OTA was detected by MS/MS in an ESI（-）-MRM mode. Results：The limit of detection was 0. 1 μg·kg^-1 , the average recoveries ranged from 84. 8% to 91. 2%, and the relative standard deviations （ RSD） ranged from 3. 6% to 8. 1%（n=9）. Conclusion：The method is accurate,sensitive and simple, and suitable for the determination of ochratoxin A in Chinese herbs. |
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| Keywords: | Chinese herbs Ochratoxin A HPLC-MS/MS Immunoaffinity column |
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