依达拉奉对大鼠脑缺血再灌注后半胱天冬酶-3的影响

目的：本试验通过观察依达拉奉对半胱天冬酶-3表达的影响,探讨它的保护作用及作用机制。方法：Wis-tar雌、雄性大鼠共42只,随机分为假手术组、盐水对照组及依达拉奉用药组,对照组及用药组再进一步分为6 h、24 h、48h。用免疫组化方法检测Caspase-3的蛋白表达的变化。结果：假手术组几乎无Caspase-3蛋白的表达,盐水组Caspase-3蛋白表达6 h开始升高,于24 h达到高峰并维持到48 h。用药组Caspase-3蛋白表达较盐水组减少,P〈0.01。结论：依达拉奉可减少大鼠脑缺血再灌注后Caspase-3蛋白的表达,达到脑保护作用。

Effect of Edaravone on Caspase-3 in rats Cerebral Ischemia Reperfusion

Objective：Edaravone is evaluated its protective effect through observing expression of Caspase-3. Methods：42 wistar rats were randomly divided into 3 groups,sham operation group,saline control group and Edaravone treatment group. Saline control group and Edaravone treatment group were divided into 6 h,24 h,48 h. The expression of Caspase-3 was investigated by Immnno-histochemistry technique. ResuLts：There was almost no expression of Caspase-3 in sham operation group. The expression of Caspase-3 in saline control group increased in 6 h and reached peak in 24 hours and continued to 48 h. Compared with that in saline control group ,the expression of Caspase-3 in Edaravone treatment group obviously reduced （P〈0.01）. Conclusion ：Edaravone could reduce the expression of Caspase-3 and protect the brain.