| 肝细胞肝癌中PED/PEA-15 mRNA及蛋白表达的临床意义 |
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| 引用本文： | 孙德光,吴益峰,高振明,梁锐,庄成君,王立明. 肝细胞肝癌中PED/PEA-15 mRNA及蛋白表达的临床意义[J]. 实用医学杂志, 2010, 26(6). DOI: 10.3969/j.issn.1006-5725.2010.06.004 |
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| 作者姓名： | 孙德光 吴益峰 高振明 梁锐 庄成君 王立明 |
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| 作者单位： | 大连医科大学附属第二医院普外三科,116027 |
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| 基金项目： | 国家高技术研究发展计划(863) |
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| 摘 要： | 目的:探讨PED/PEA-15 mRNA及蛋白在肝癌组织、癌旁组织及正常肝组织中的表达情况及其临床意义.方法:应用半定量逆转录.聚合酶链反应检测40例肝细胞肝癌、相应癌旁组织和12例正常肝组织中PED/PEA.15基因的相对表达量.并通过免疫组化检测PED/PEA-15蛋白的表达.结果:半定量RT-PCR显示,PED/PEA-15基因在肝癌组织中均呈高表达,癌旁组织及正常肝组织呈低表达或不表达,相对表达量分别为1.201±0.301、0.64±0.101和0.565±0.077,PED/PEA-15 mRNA在肝癌组织中表达较癌旁组织、正常肝组织显著升高(P<0.01).免疫组织化学显示,肝癌组织、癌旁组织及正常肝组织中PED/PEA-15蛋白的表达率分别为72.5%、32.5%和16.7%.肝癌组织中PED/PEA-15蛋白表达较癌旁组织及正常肝组织显著升高(P<0.01).且PED/PEA-15 mRNA及蛋白的表达与肝癌病理分级、临床分期有关(P<0.05),而与发病年龄、性别、肿瘤大小、肿瘤数目差异无显著性(P>0.05).结论:PED/PEA-15 mRNA及蛋白在肝癌组织中表达水平明显升高,可能在肝癌的发生及发展过程中发挥重要作用.
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| 关 键 词： | 癌,肝细胞 逆转录聚合酶链反应 免疫组化 |
Expression and significance of PED/PEA-15 mRNA in hepatocellular carcinoma |
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| SUN De-guaug,WU Yi-feng,GAO Zhen-ming,LIANG Rui,ZHUANG Cheng-jun,WANG Li-ming. Expression and significance of PED/PEA-15 mRNA in hepatocellular carcinoma[J]. The Journal of Practical Medicine, 2010, 26(6). DOI: 10.3969/j.issn.1006-5725.2010.06.004 |
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| Authors: | SUN De-guaug WU Yi-feng GAO Zhen-ming LIANG Rui ZHUANG Cheng-jun WANG Li-ming |
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| Affiliation: | SUN De-guang,WU Yi-feng,GAO Zhen-ming,LIANG Rui,ZHUANG Cheng-jun,WANG Li-ming.The Third Department of General Surgery,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China |
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| Abstract: | Objective To investigate the expression of PED / PEA-15 mRNA and protein in hepatocellular carcinoma (HCC) tissue,para-carcinoma tissue and normal hepatic tissue and its clinical significance.Methods The expression of PED / PEA-15 mRNA was detected by RT-PCR,and the expression of PED / PEA-15 protein was measured by immunohistochemical (IHC) ElivisonTM plus non-biotin technique in 40 cases of HCC,40 cases of corresponding para- carcinoma tissue,and 12 cases of normal hepatic tissue.Results The expression of... |
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| Keywords: | PED/PEA-15 |
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