川芎嗪对大鼠支气管哮喘模型气道重塑的影响及机制

目的　观察川芎嗪对支气管哮喘 (简称哮喘 )大鼠模型气道重塑的抑制作用并探讨其作用机制。方法　 32只SD大鼠按随机数字表法分成正常对照组 (A组 )、哮喘模型组 (B组 )、小剂量川芎嗪组 (C组 ,4 0mg/kg)和大剂量川芎嗪组 (D组 ,80mg/kg) ,以卵白蛋白 (OVA)致敏并长期吸入激发制备大鼠慢性哮喘模型。采用免疫组化半定量法测定气道壁胶原和转化生长因子 β1(TGF β1)含量 ,同时测定气道内、外径及平滑肌层、网状基底膜的厚度。结果　D组气道平滑肌层、网状基底膜的厚度为 (11 3± 1 3) μm、(11 3± 1 7) μm ,B组分别为 (19 7± 1 8) μm、(19 8± 1 6 ) μm ,两组比较差异有统计学意义 (P均 <0 0 1) ,但D组与A组 (10 6± 1 2 ) μm、(9 8± 1 6 ) μm]、C组 (11 6± 0 9) μm、(12 3± 1 8) μm]比较差异无统计学意义 (P均 >0 0 5 ) ;D组气道内外径比值为 0 77± 0 0 6 ,B组为0 6 3± 0 0 5 ,D组与B组比较差异有统计学意义 (P <0 0 1) ;D组气道壁Ⅲ型胶原及TGF β1含量吸光度 (A)值分别为 2 1± 5、2 6± 5 ,B组分别为 5 5± 7、6 9± 14 ,两组比较差异有统计学意义 (P <0 0 1) ,D组与C组 (32± 8、38± 10 )比较差异有统计学意义 (P <0 0 5 ) ,C组与B组比较差异也有统计学意义 (P <0 0

Effect of ligustrazine on airway remodeling in asthmatic rats

OBJECTIVE: To observe the effect and mechanism of ligustrazine on the airway remodeling. METHODS: Thirty-two SD rats were randomly divided into 4 groups: the normal group (A), the model group (B), the ligustrazine low-dose group (C, 40 mg/kg) and the ligustrazine high-dose group (D, 80 mg/kg), with 8 rats in each group. The chronic asthmatic model was established by repeated inhalation of ovalbumin. The changes of collagen and transforming growth factor-beta(1) (TGF-beta(1)) contents in the airway wall, the thickness of smooth muscle and basement membrane, inner and outer diameter were measured by the computerized image analysis system. RESULTS: The thickness of smooth muscle and basement membrane were (11.3 +/- 1.3, 11.3 +/- 1.7) microm in D group, (19.7 +/- 1.8, 19.8 +/- 1.6) microm in B group, the difference being significant (P < 0.01), as compared with A group (10.6 +/- 1.2) microm, (9.8 +/- 1.6) microm] and C group (11.6 +/- 0.9) microm, (12.3 +/- 1.8) microm], the difference being not significant (all P > 0.05). The difference in the ratio of inner diameter to outer diameter was significant between D group (0.77 +/- 0.06) and B group (0.63 +/- 0.05), P < 0.01. The contents of collagen type III and TGF-beta(1) were (21 +/- 5, 26 +/- 5) in D group, (55 +/- 7, 69 +/- 14) in B group, the difference being significant (P < 0.01). The differences were also significant when C group 32 +/- 8, 38 +/- 10] was compared with D group (P < 0.05) and B group (P < 0.01). The contents of collagen type I showed no difference among the 4 groups (A group: 34 +/- 13, B group: 44 +/- 8, C group: 36 +/- 8, D group: 39 +/- 8; all P > 0.05). A close correlation between TGF-beta(1) and collagen type III was demonstrated (r = 0.844 2, P < 0.01). CONCLUSION: Ligustrazine might suppress airway remodeling by decreasing the expression of TGF-beta(1) and reducing deposition of collagen.