人工泪液对大鼠视网膜电图的影响

目的 观察人工泪液对大鼠视网膜电图（ERG）的影响.方法 动物实验研究.SD大鼠充分暗适应后,记录单眼ERG.ERG记录采用自制银丝电极和不锈钢针状电极,其中银丝电极为记录电极,置于角巩膜缘,各组ERG记录眼人工泪液用量不同：第1组为未滴人工泪液组；第2组为适量组,滴少量人工泪液,使电极与角巩膜缘接触良好；第3组为过量组,滴入大量人工泪液,使之充满整个结膜囊.ERG a、b波幅值和峰时采用单因素方差分析比较组间差异,两两间差异采用LSDt法进行比较.结果 使用人工泪液的两组动物ERG基线平滑,干扰波不明显；然而人工泪液使用过量组a、b波振幅均明显降低,与其他两组相比差异均有统计学意义（t=4.112,P＜0.01；t=3.018,P＜0.05）.结论 视觉电生理实验室经屏蔽和有效接地后,动物ERG记录中使用人工泪液可以进一步降低干扰,但人工泪液使用过量会影响ERG振幅大小.

Influence of artificial tear solution on the electroretinogram of adult rats

Objective To observe the effect of artificial tear solution on the electroretinogram (ERG) of SD rats.Methods Fifteen adult SD rats were divided into 3 groups in this experimental study.After 6 hours of dark adaptation,a silver loop electrode was placed in contact with the cornea as the recording electrode. Stainless steel needles were used as reference and ground electrode.Different amounts of artificial tear (1％ carboxymethylcellulose sodium) were instilled in the recording eye: group one: the cornea was kept dry without artificial tear; group two: the amount of artificial tear was moderate; group three: the amount was excessive. ERG was recorded and the a- and b-waves were monitored.Data were analyzed with a one-way ANOVA and differeces between each group were compared by the LSD t test.Results In groups two and three, the interference was subtle and the baseline was smooth compared to group one. There was a statistically significant decrease in the a- and b-wave amplitudes in group three (t=4.112, P＜0.01; t=3.018,P＜0.05).Conclusion Artificial tear solution can reduce interference in ERG recordings but excessive amounts may affect the amplitude of the waves.