Evaluation of a passive microcapsule agglutination test for the screening of human leptospirosis |
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Authors: | B. Cacciapuoti L. Ciceroni Y. Arimitsu T. Sato M. Seki |
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Affiliation: | (1) National Center for Leptospirosis, Istituto Superiore di Sanità, V.le Regina Elena, 299, 00161 Rome, Italy;(2) Department of Applied Immunology, National Institute of Health, Tokyo, Japan;(3) Japan Lyophilization Laboratories, Tokyo, Japan |
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Abstract: | Microcapsules, absorbed with two mixed antigens- each composed of 3 sonicated leptospira serovars — were developed in the past as a microcapsule agglutination test (MCA-LS) for the screening of clinical leptospirosis.Fof this study, fifty serum samples, taken at an earlier and at a later stage of illness from 25 Italian in-patients with clinical symptoms of leptospirosis, were tested with both the MCA-LS one-dilution test and the microscopic agglutination (MA) test, the confirmatory test for leptospirosis, with 18 leptospira strains circulating in Italy. Compared with MA, MCA-LS showed a sensitivity of 91.7%, and a specificity of 92.3%, including leptospiroses not substained by the diagnostic strains used in the MCA-LS kit. |
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Keywords: | Leptospirosis Serological screening Microcapsule agglutination Passive agglutination |
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