兔儿伞总黄酮抗炎作用研究

目的:观察兔儿伞总黄酮的抗炎作用.方法:以二甲苯致小鼠耳肿胀模型、醋酸致小鼠腹腔毛细血管通透性增加模型,棉球诱发小鼠肉芽肿及小鼠气囊炎模型,观察兔儿伞总黄酮对不同炎症模型的抗炎作用.动物均分为模型(生理盐水)组、地塞米松(2.0 mg· kg-1)组、兔儿伞总黄酮高、中、低(400,200,100 mg·kg-1)组,1次/d,灌胃给药,连续7d.结果:兔儿伞总黄酮各剂量组能显著降低小鼠毛细血管通透性(P＜0.01)；兔儿伞总黄酮高剂量组对二甲苯致小鼠耳肿胀有抑制作用,肿胀抑制率51.55％ (P ＜0.01)；兔儿伞总黄酮能减轻小鼠肉芽肿,兔儿伞总黄酮各剂量组气囊渗出液中前列腺素E2(PGE2)和白细胞数含量低于对照组(P＜0.01).结论:兔儿伞总黄酮具有较强的抗炎作用,其抗炎作用可能与其降低血管通透性、抑制PGE2的生物合成或释放有关.

Experimental Anti-inflammatory Activity of Total Flavonoids from Syneilesis aconitifolia

Objective:To investigate the anti-inflammatory activity of total flavonoids from Syneilesis aconitifolia. Method: Mice were involved in the study. The auricle edema was induced by dimethylbenzene, the abdominal cavity capillary permeability was increased by acetic acid, the granuloma was induced with cotton pellet, and the acute inflammation was induced by air-pouch. The anti-inflammatory effect of total flavonoids in different models were observed. Mice were divided into five groups, total flavonoids from S. acontifolia high dose, middle dose and low dose (400,200, 100 mg·kg^-1) groups, dexamethasone (2.0 mg·kg^-1) group and the model group (normal saline). The drug treatment lasted 7 days. Result: The total flavonoids from S. acontifolia showed significant inhibitory action on auricle edema induced by dimethylbenzene in mice (P<0.01), the inhibitory rate was 51.55%. The total flavonoids from S. acontifolia showed significant inhibitory action on penetration of capillary vessel in mice (P<0.01). The flavonoids also decreased the proliferation of granuloma caused by implantation cotton pellet in mice significantly. The amount of WBC and content of prostaglanding E₂(PGE₂) in the exudates in experimental groups were obviously lower than those in the control group (P<0.01). Conclusion: The total flavonoids from S. aconitifolia have significant anti-inflammation action, which is likely related to the decrease in capillary permeability and inhibition of production and release of the inflammatory factor PGE₂.