颈椎间盘髓核组织中TRAIL及DR5的表达与髓核细胞凋亡的相关性研究

目的检测颈椎间盘内髓核组织细胞密度、细胞凋亡率以及肿瘤坏死因子相关凋亡诱导配体（TRAIL）及死亡受体5（DR5）的表达。方法实验组30个标本来自脊髓型颈椎病患者前路手术所切取的椎间盘组织（C3-C7）,对照组30个标本为颈椎创伤患者前路手术所取得的椎间盘组织。采用HE染色观察髓核组织中凋亡小体和细胞密度,原位DNA片段末端标记法（TUNEL）检测凋亡细胞率,用免疫组织化学法检测TRAIL、DR5的表达。结果实验组髓核组织中的细胞密度均低于对照组,TUNEL阳性细胞率均高于对照组（P〈0.01）;实验组TRAIL、DR5染色阳性细胞率均高于对照组（P〈0.05）。将两组全部60例颈椎间盘标本合并后进行Pearson相关分析,颈椎间盘TUNEL阳性细胞率与细胞密度之间呈负相关（r=-0.969,P〈0.01）;两组髓核内TRAIL、DR5染色阳性细胞率与TUNEL阳性细胞率之间呈正相关r=0.921和0.755,P〈0.01）。结论细胞密度下降参与了颈椎间盘的退变过程,细胞凋亡是椎间盘髓核细胞减少的原因之一,颈椎间盘组织中存在DR5/TRAIL诱导的凋亡途径。

Study on the expression of TRAIL and TRAIL-R2 (DR5) in the pulpy nucleus tissue of human cervical disc and the apoptosis of nuclei pulposus

Objective To determine the cell density,apoptosis rate and the expressions of TRAIL and DR5 in human cervical intervertebral discs.Methods The control group consisted of 30 human with traumatic normal cervical intervertebral discs(C3-C7)and 30 human with degenerative cervical intervertebral discs in experimental group(C3-C7)were surgically collected from patients undergoing anterior cervical discectomy and fusion(ACDF).Apoptotic bodies and the cell density were detected by HE staining;Apoptotic disc cells w...