P-选择素基因多态性与冠心病的相关性

目的研究P-选择素基因多态性与冠心病（coronaryheartdisease，CHD）易感性之间的相关性。方法以2012年6月-2013年4月辽宁医学院附属第-医院心内科病房的200例冠心病患者为病例组，210例健康体检者为对照组，应用聚合酶链反应-限制性片段长度多态性（polymerasechainreaction—restrictionfragmentlengthpolymorphism，PCR—RFLP）方法和DNA测序法，检测两组人群P-选择素基因启动子区-2123C／G、-1969G／A和-1817T／C的基因多态性，并将冠心病组分为心绞痛、心肌梗死和缺血性心力衰竭组，研究不同类型冠心病组与对照组之间P-选择素基因启动子区-2123C／G、-1969G／A和-1817T／C位点基因多态性分布的差异。结果P-选择素基因-2123C／G位点基因型和等位基因频率在冠心病组和对照组间的分布差异有统计学意义（x2=6．124，P=0．047；x2=7．355，P=0．008），并且在心肌梗死组与对照组、缺血性心力衰竭组与对照组的分布差异也具有统计学意义（x2=6．473，P=0．039；x2=13．944，P=0．001）。-1817T／C和-1969G／A位点基因型和等位基因频率在冠心病组和对照组间以及不同类型冠心病组和对照组间的分布差异均无统计学意义俨〉0．051。结论P-选择素基因-2123C／G位点多态性与冠心病易感性之间具有相关性。

Correlation between P-selectin gene polymorphism and coronary heart disease

Objective To study the correlation between P-selectin gene polymorphism and coronary heart disease（CHD）. Methods Two hundred CHD patients admitted to our hospital from June 2012 to April 2013 served as a CHD group and 210 subjects undergoing physical examination served as a control group in this study. Polymorphism of their P-selectin genes -2123C/G,-1969G/A and -1817T/C was detected by PCR-RELP and DNA sequencing, respectively. CHD patients were further divided into angina pectoris group, myocardial infarction group and ischemic heart failure group. Difference in polymorphism of P-selectin genes -2123C/G, -1969G/A and -1817T/C was observed in different types of CHD. Results The frequency of P-selectin gene -2123C/G and allelic gene was significantly higher in CHD group than in control group（x 2=6.124, P=0.047; X 2=7.355, P=0.008） and in myocardial infarction group and ischemic heart failure group than in control group（x 2=6.473, P=-0.039; X 2=13-944, P=0.001）. However, no significant difference was found in the frequency of P-selectin genes -1817T/C and -1969G/A and allelic gene between CHD group and control group（P 〉 0.05）. Conclusion P-selectin gene -2123C/G polymorphism is correlated with CHD.