铜绿假单胞菌LCT-PA220菌株全基因组序列测定与分析

目的全基因组测序和分析铜绿假单胞菌LCT—PA220菌株，研究空间因素对细菌全基因组的影响，为宇航员的保健奠定基础。方法通过革兰染色鉴定LCT—PA220属性，提取基因DNA、构建文库并上机测序；加工处理原始测序数据后进行基因组组装、注释和分析。结果铜绿假单胞菌LCT—PA220菌株革兰染色阴性，基因组为6．87M，包括5829个编码序列，平均长度为962bp，预测到为54个tRNA基因，COG数据库和KEGG数据库比对共注释到22组COG功能和32条KEGG信号通路。结论铜绿假单胞菌LCT—PA220菌株全基因组测序和基因组清楚，为后续对该菌株的研究打下了基础。

Whole genome sequencing and analysis of Pseudomonas aeruginosa strain LCT-PA220

Objective To study the influence of the spatial factor which is closely associated with spacemen＇s health, the whole genome sequence of Pseudomonas aeruginosa strain LCT-PA220. Methods The characteristic was identified by gram stain examination. Then genomic DNA was extracted from LCT-PA220 strain and the library was constructed and followed by sequencing. The raw date were filtered, assembled into genome, annotated and analyzed. Results The identify of LCT-PA220 strain was gram-negative bacteria whose genome was 6.87M containing 54 tRNA genes and 5 829 coding sequences with 962 bp average gene length. COG and KEGG annotation analysis revealed 22 COG functions and 32 KEGG pathways, respectively. Conclusion The whole genome of LCT-PA220 strain is sequenced and annotated well, which paves the way for further study of this strain.