骨松康含药血清对成骨细胞生物学活性的影响

目的 观察骨松康对体外培养成骨细胞增殖和分化的影响。方法 分离培养乳鼠颅骨成骨细胞，加入中药骨松康吸收后血清，流式细胞仪和碱性磷酸酶活性检测、骨钙素免疫组化染色、矿化结节计数分析其对成骨细胞增殖和分化的影响。结果以含药血清培养的成骨细胞，S期细胞比例增多，说明细胞DNA的合成增加；碱性磷酸酶活性及骨钙素表达增强；形成矿化结节数及分泌胰岛素样生长因子1（IGF-1）的含量均明显高于对照血清组（P〈0．01）。结论 骨松康含药血清能直接促进体外培养的大鼠成骨细胞的增殖、分泌和矿化，提高了成骨细胞骨形成功能。

Effects of serum containing traditional Chinese medicine on proliferation and differentiation of osteoblasts in vitro

Objective To study effects of serum containing traditional Chinese medicine on proliferation and differentiation of osteoblasts in vitro. Methods Osteoblasts were isolated from the skull of 3-7 day newly born rats and the medium was changed for that containing serum with absorbed Gusongkang. Proliferation and differentiation of osteoblasts were analyzed by flowcytometry and ALP activity analysis and osteocalcin immunohistochemical staining and number of calcium nodule. Results In the cells cultured by serum containing medicine, cellular proportion in S period increased, which indicates the increase of synthesis and propagation of cell DNA; ALP activity and osteocalcin expression significantly increased. The number of calcium nodule formed osteoblasts cultured by the serum fed with Gusongkang higher than that osteoblasts cultured by control serum (P<0.01). Conclusion The serum from rats fed with Gusongkang can directly promote the proliferation and scretion and mineralization of cultured osteoblasts in vitro.