急性白血病免疫表型的预后意义

免疫表型对白血病的预后判断有重要意义。目前临床宜选用一些与细胞分化阶段有明确关系的、代表某些形态学特征的抗原进行免疫分型,可补充FAB分型的不足,还可确定形态学不能或很难区分的白血病类型。通过免疫表型检测和计分可明确双表型白血病和未分化白血病的诊断。某些特异的抗原与特异的细胞遗传学以及分子水平异常有关,决定了白血病的恶性行为及对治疗的反应。用多参数流式细胞术可同时检测2个或更多的抗原,数种抗原组合可用来检测微小残留病,提高检测的准确性,在患者初诊时即对其各白血病细胞亚群进行表型分析,为以后的微小残留病监测提供依据,才具有预测复发的价值。

Significance of Immunophenotyping in Prognosis of Acute Leukemia

Immunophenotyping is significantly important in the determination of prognosis of acute leukemia patients. Acute leukemia may be classified by several cellular-stage-specific antigens, which are also representative of certain morphologic characterizations. Immunophenotyping may assist FAB classification and define some subtypes that are difficult to identify solely by morphologic examinations. Exact diagnosis of biphenotypic and undiff eren-tiated acute leukemias may be attained by Immunophenotyping and its scoring system. In addition, expression of specific phenotype is associated with certain cytogenetic and molecular abnormalities, which underlies the malignant behavior of leukemias and the response to therapy. Moreover, combination of several antigens is a novel approach in monitoring minimal residual disease. Multiparameter flow cytometry may detect two or more antigens simultaneously. Since different subpopulations of leukemia cells may coexist in the same patient, the original data of immunophenotype should be preserved in order to help identify minimal residual disease later.