| 单细胞凝胶电泳技术及其应用进展 |
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| 引用本文： | 邢彩虹,李桂兰,尹松年.单细胞凝胶电泳技术及其应用进展[J].卫生研究,2004,33(5):638-640. |
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| 作者姓名： | 邢彩虹 李桂兰 尹松年 |
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| 作者单位： | 中国疾病预防控制中心职业卫生与中毒控制所,北京,100050 |
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| 摘 要： | 单细胞凝胶电泳又称彗星试验 ,是一种在单细胞水平上检测DNA单、双链断裂和碱性不稳定位点的方法 ,具有敏感、稳定、快速的优点。近些年来 ,该方法广泛应用于DNA修复、遗传毒理、环境监测和细胞凋亡的研究。本文就彗星试验结合荧光原位杂交、检测DNA碱基氧化损伤、以及在染色、玻片储存和彗星图像分析方面的进展进行了综述
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| 关 键 词： | 单细胞凝胶电泳 DNA损伤 |
| 文章编号： | 1000-8020(2004)05-0638-03 |
| 修稿时间： | 2003年11月25 |
Single cell gel electrophoresis assay-applications and progress |
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| Xing Caihong,Li Guilan,Yin Songnian Institute for Occupational Health and Poison Control,China CDC,Beijing ,China.Single cell gel electrophoresis assay-applications and progress[J].Journal of Hygiene Research,2004,33(5):638-640. |
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| Authors: | Xing Caihong Li Guilan Yin Songnian Institute for Occupational Health and Poison Control China CDC Beijing China |
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| Institution: | Institute for Occupational Health and Poison Control, China CDC, Beijing 100050, China. |
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| Abstract: | The single cell gel electrophoresis or Comet assay is a sensitive, reliable, and rapid method for DNA double- and single-strand breaks and alkali-labile sites, in eukaryotic individual cells. In recent years, this method has been widely used for studies on DNA repair, genetic toxicology, environmental biomonitoring and apoptosis. In this paper, the progress of the assay in:(1) fluorescence in situ hybridization with comet, (2)the use of repair enzymes to detect specific classes of DNA damage, and (3)DNA-specific dye, slide storage and analysis of comet were reviewed. |
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| Keywords: | single cell gel electrophoresis DNA damage |
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