鼻咽癌患者外周血淋巴细胞的金属元素与T细胞亚群的研究

目的 :探讨鼻咽癌患者外周血淋巴细胞金属元素和T细胞亚群含量的变化及其与机体免疫功能的关系。方法 :采用日本岛津AA 6 70型原子吸收光谱仪火焰法测定鼻咽癌组和正常对照组外周血淋巴细胞金属元素的含量 ,流式细胞仪测定T细胞亚群。结果 :鼻咽癌组淋巴细胞铜含量显著高于正常对照组 (P <0 .0 1) ,锌含量低于正常对照组 (P <0 .0 5 ) ;鼻咽癌组CD3 、CD4细胞和CD4/CD8比值明显低于正常对照组 (P <0 .0 1)。结论 :鼻咽癌患者外周血淋巴细胞铜含量增高时 ,CD4细胞数量减少 ,表明CD4细胞的克隆发育功能受到影响 ,从而揭示鼻咽癌患者外周血淋巴细胞铜含量的变化与T细胞亚群变化的关系

Study on some metal elements levels and the T lymphocyte subset of the peripheral lymphocytes in patients with nasopharyngeal carcinomas

OBJECTIVE: To study some metal elements levels and T lymphocyte subset of the peripheral lymphocytes in patients with nasopharyngeal. METHOD: Metal elements in the patients with nasopharyngeal and normal control were detected by the Atomic Absorption Spectrometer (Flame SHIMATZV.JAPAN). T lymphocyte subset were detected by the flow cytometry (FCM). RESULT: The level of copper was significantly higher and the level of zinc was significantly lower in patients with nasopharyngeal carcinomas than those in normal control group (P < 0.01 and < 0.05 respectively). The number of CD3/CD4 cell in the patients group were less than those in normal group(P < 0.01). CONCLUSION: The results of correlative analysis showed that there was a significant correlationship between the number of CD4 cells and levels of copper in peripheral lymphocytes of the patients group.