| Abstract: | Objective: To evaluate sperm chromosome aneuploidy and DNA fragmentation in infertile couples with unexplained recurrent miscarriage (URM).Methods: Multi-color fluorescence in situ hybridization (FISH) protocol was performed with the probes specific for the chromosomes 13, 18, 21, X and Y in the control group (n= 5) and the infertile patients with URM (n=12). The level of DNA fragmentation was determined by Sperm Chromatin Dispersion (SCD) test in the patients with URM (n=48) and the fertile men (control group, n=32).Results: The patients with URM had total numerical abnormality rate of 3.91% (n=12) for the chromosomes 13, 18 and 21 and 1.98% for the chromosomes X and Y respectively, which significantly higher than that of the control group (1.29% and 0.61%, n = 5, P<0.01). A proportion of total sperm DNA fragmentation was detected in infertile patients with URM (2.1±10.3%) (n = 48), which significantly higher than the control group(2.1±5.2%, P<0.01).Conclusions: Spermatozoa from couples with URM contained high rates of DNA fragmentation and chromosomal aneuploidy. Screening for sperm chromosomal aneuploidy and sperm DNA fragmentation may provide the useful information for investigating male unexplained infertility. |
