| Survivin反义寡核苷酸转染对肝门部胆管癌细胞FRH-0201侵袭性的影响 |
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| 引用本文： | 赵蒙,智绪亭.Survivin反义寡核苷酸转染对肝门部胆管癌细胞FRH-0201侵袭性的影响[J].中国现代普通外科进展,2010,13(1):5-8. |
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| 作者姓名： | 赵蒙 智绪亭 |
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| 作者单位： | 1. 山东大学齐鲁医院,普外科,山东,济南,250012;山东大学医学院,山东,济南,250012
2. 山东大学齐鲁医院,普外科,山东,济南,250012 |
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| 摘 要： | 目的：观察Survivin反义寡核苷酸转染对肝门部胆管癌细胞FRH-0201侵袭能力的影响。方法：针对Survivin基因序列设计合成反义寡核苷酸,阳离子脂质体包裹转染胆管癌细胞株FRH-0201。观察转染后癌细胞生长增殖情况,酶联免疫吸附试验检测细胞上清液中基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶抑制因子-2（TIMP-2）含量,侵袭小室检测细胞侵袭能力的改变。结果：反义转染组细胞生长及增殖减缓并趋向凋亡,细胞上清液中MMP-2因子较正义转染组及阴性对照组少,且差异有统计学意义（P〈0.05）,TIMP-2因子较另2组有所增加,且差异有统计学意义（P〈0.05）,Transwell实验中反义转染组穿过小室细胞数目较另2组减少（P〈0.05）。结论：Survivin反义寡核苷酸转染可有效抑制肝门部胆管癌细胞增殖,并降低癌细胞的侵袭能力。
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| 关 键 词： | Survivin寡核苷酸类 反义 肝门部胆管肿瘤 侵袭性 基质金属蛋白酶-2 |
Effect of survivin antisense oligodeoxynucleotide on invasive ability of hilar cholangiocarcinoma cell FRH-0201 |
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| ZHAO Meng,ZHI Xu-ting.Effect of survivin antisense oligodeoxynucleotide on invasive ability of hilar cholangiocarcinoma cell FRH-0201[J].Chinese Journal of Current Advances in General Surgery,2010,13(1):5-8. |
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| Authors: | ZHAO Meng ZHI Xu-ting |
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| Institution: | Jinan 250012/a>;China;Jinan 250012/a>;China |
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| Abstract: | Objective: To study the inhibition effect of survivin antisense oligodeoxynucleotide (ASODN)on apoptosis and invasion of hilar cholangiocarcinoma cell line FRH-0201. Methods: ASODN was transfected into cholangiocarcinoma cell line FRH-0201 by LipofectamineTM2000. The level of MMP-2 and TIMP-2 in the supernatant was detected by enzyme linked immunosorbent assay (ELISA). The effect of ASODN transfection was observed by Transwell cell culture chamber. Results: The cell proliferation was inhibited in the ASODN ... |
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| Keywords: | Survivin antisense oligodeoxynucleotides·Hilar cholangiocarcinoma·Invasive·Matrix metalloproteinase-2 |
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