Effects of brazilin on the phospholipase A2 activity and changes of intracellular free calcium concentration in rat platelets |
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| Authors: | Gwi-Seo Hwang Ji-Young Kim Tong-Shin Chang Sun-Duck Jeon Dhong-Su So Chang-Kiu Moon |
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| Institution: | (1) College of Oriental Medicine, Kyung Won University, Seongnam, Korea;(2) College of Pharmacy, Seoul National University, Shilim-Dong, Kwanak-Gu, 151-742 Seoul, Korea |
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| Abstract: | Brazilin {7,11 b-dihydrobenzb]indeno1,2-d]pyran-3,6a,9,10(6H)-tetrol} inhibited thrombin-, collagen-and ADP-induced aggregation of washed rat platelets. Thrombin- and collagen-induced
ATP release were also inhibited by brazilin in a concentration-dependent manner. Brazilin inhibited the formation of platelet
thromboxane A2 caused by thrombin, whereas it had no effect on the prostaglandin D2 formation. Brazilin inhibited 3H]-arachidonic acid liberation from membrane phospholipids of thrombin-stimulated platelets. Brazilin inhibited the rise of
intracellular free calcium caused by thrombin. These results indicate that the inhibition of phospholipase (PLA2) activity and Ca2+]i elevation might be at least a part of antiplatelet mechanism of brazilin. |
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| Keywords: | Brazilin Caesalpinia sappan Aggregation Phospholipase A2 Platelet Intracellular free calcium |
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