| 人衰老成纤维细胞凋亡的可诱导性 |
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| 引用本文： | 黄英,张宗玉,童坦君.人衰老成纤维细胞凋亡的可诱导性[J].中华老年医学杂志,2000,19(3):170-173. |
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| 作者姓名： | 黄英 张宗玉 童坦君 |
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| 作者单位： | 北京大学医学部生物化学与分子生物学系 |
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| 基金项目： | 国家自然科学基金!重点资助项目 ( 3 993 0 170 )，国家重点基础研究发展规划资助!项目 ( G19990 5 3 90 6) |
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| 摘 要： | 目的 研究体外培养的人成纤维细胞衰老时可诱发凋亡的能力。方法 用于酸钠和去血清两种方法诱导人衰老和年轻或纤维细胞生存曲线、细胞形态和超微结构、ＤＮＡ断裂电科 、流式细胞仪分析凋亡等手段检测凋亡的发生情况。结果 ５０ｍｍｏｌ／Ｌ的丁酸钠诱导年轻细胞４８ｈ即发生凋亡，而老细胞耐９６ｈ，去血清诱导年轻细胞１５ｄ左右发生凋亡，而衰老细胞此时未出现凋亡现象。结论 人老成纤维细胞可凋亡的能力明显低于年轻细胞。
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| 关 键 词： | 成纤维细胞 衰老 细胞凋亡 |
The ability of inducing apoptosis in human senescent fibroblasts |
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| HUANG Ying,ZHANG Zongyu,TONG Tanjun.The ability of inducing apoptosis in human senescent fibroblasts[J].Chinese Journal of Geriatrics,2000,19(3):170-173. |
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| Authors: | HUANG Ying ZHANG Zongyu TONG Tanjun |
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| Institution: | HUANG Ying,ZHANG Zongyu,TONG Tanjun. Department of Biochemistry and Molecular Biology,Beijing Medical University,Beijing 100083,China |
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| Abstract: | Objective To study the ability of inducing apoptosis in senescent human fibroblasts cultured in vitro. Methods Senescent and young human fibroblasts were induced by sodium butyrate (NaBu) or serum deprivation. Apoptosis was observed from analysis of cell survival curve, cell morphological and ultrastructural changes, DNA fragmentation electrophotogram and flow cytometry. Results 50 mmol/L NaBu induced apoptosis in young cells after 48 hours; while in senescent cells it happened after 96 hours. Serum deprivation induced apoptosis in young cells after about 15 days; while in senescent cell, no apoptotic phenomena showed at this time. Conclusions The ability of inducing apoptosis in senescent human fibroblasts is apparently lower than that in young ones. |
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| Keywords: | Fibroblasts Aging Apoptosis |
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