| bFGF对骨肉瘤细胞系U2-os细胞的促凋亡作用 |
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| 引用本文： | 彭博,吴哲,张朝东.bFGF对骨肉瘤细胞系U2-os细胞的促凋亡作用[J].中国医科大学学报,2007,36(5):526-528. |
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| 作者姓名： | 彭博 吴哲 张朝东 |
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| 作者单位： | 1. 中国医科大学门诊部,辽宁,沈阳,110001
2. 中国医科大学附属第一医院神经内科,辽宁,沈阳,110001 |
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| 摘 要： | 目的:观察碱性成纤维细胞生长因子(bFGF)对骨肉瘤细胞系U2-os细胞增殖及生存的影响,探讨bFGF在肿瘤细胞发生发展中的作用。方法:以bFGF处理U2-os细胞,采用台盼蓝排除检测法及MTT法检测细胞存活及增殖情况;用Annexin V-EGFP/PI双染流式细胞术及DNA梯状带检测细胞凋亡情况。结果:bFGF呈浓度、时间依赖性降低U2-os细胞活力并诱导细胞凋亡。结论:bFGF可以诱导U2-os细胞发生凋亡,生长因子在细胞的生长调控方面具有双重作用。
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| 关 键 词： | 骨肉瘤细胞系U2-os细胞 碱性成纤维细胞生长因子 凋亡 |
| 文章编号： | 0258-4646（2007）05-0526-03 |
| 修稿时间： | 2007-07-13 |
Effect of bFGF on the apoptosis of U2-os osteosarcoma cell line |
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| PENG Bo,WU Zhe,ZHANG Chao-dong.Effect of bFGF on the apoptosis of U2-os osteosarcoma cell line[J].Journal of China Medical University,2007,36(5):526-528. |
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| Authors: | PENG Bo WU Zhe ZHANG Chao-dong |
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| Institution: | 1 .Department of Clinic, China Medical University, Shenyang 110001, China; 2.Department of Neurology , The First Affiliated Hospital |
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| Abstract: | Objective:To study the role of basic fibroblast growth factor (bFGF) in the generation and development of tumor by investigating the inhibition effect of bFGF on the proliferation and survival of U2-os osteosarcoma cell line. Methods:U2-os cells were treated by bFGF,and their survival and proliferation were determined with trypan blue exclusion method and MTT method. The apoptosis in U2-os induced by bFGF was determined by Annexin V-EGFP/PI staining of fixed cells and with FACS can flow cytometry and DNA ladder analysis. Results:bFGF decreased cell activity and proliferation and induced apoptosis in U2-os cells in dependency of concentration and time. Conclusion:bFGF can induce the apoptosis of U2-os osteosarcoma cell line,and the growth factors have double regulative effects on cell growth. |
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| Keywords: | U2-os osteosarcoma cell line basic fibroblast growth factor apoptosis |
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