实验性PVR中脂质过氧化反应及玻璃体内注入维生素E对其作用的研究

目的 :研究实验性增生性玻璃体视网膜病变 (PVR)形成过程是否与自由基引发的脂质过氧化反应密切联系以及玻璃体内注射维生素E是否对PVR形成过程中发生的脂质过氧化反应产生抗氧化作用。方法 :18只健康家兔行气体压迫玻璃体术 ,3d后分三组 (每组 12只兔眼 )行气液交换。模型对照组给予 0 .5mL平衡盐溶液 ;5 0 μg维生素E组给予 0 .5mL含 5 0 μg维生素E的平衡液溶液 ;10 0 μg维生素E组给予 0 .5mL含 10 0 μg维生素E的平衡液溶液。同时 ,三组玻璃体内还注入 0 .1mL含 2 .5× 10 5的成纤维细胞悬液。气液交换后 15d及 30d分别从三组中随机各取 6只兔眼 ,抽取 0 .6mL玻璃体 ,并另取 6只正常兔眼作为正常对照组 ,也抽取 0 .6mL玻璃体 ,然后测量脂质过氧化物(LPO)及超氧化物歧化酶 (SOD)的含量。结果 :模型对照组在PVR模型建立 15d及 30d ,玻璃体内LPO及SOD较正常对照组明显升高 (P <0 .0 1)。当玻璃体内注入维生素E后 ,两给药组的LPO较模型对照组明显降低 (P <0 .0 1) ,SOD亦降低 (P <0 .0 5 )。结论 :PVR形成过程中存在着显著的脂质过氧化反应 ,玻璃体内注入维生素E能抑制PVR形成过程中的脂质过氧化反应 ,发挥强大的抗氧化作用

Lipid peroxidation in a rabbit model PVR and effect of intravitreal a-tocopherol on it

Objctive:To probe into the relationship between PVR and lipid peroxidation,and evaluate the ability of a-tocopherol to inhibit lipid peroxidation in PVR.Methods:eighteen rabbits underwent gas-compression vitrectomy.After three days,they were divided into 3 groups and underwent gas/fluid exchange.The model control group:0.5 mL 0.57 % ethamol in balaced salt solution.50 μg Vite group:50 μg a-tocopheral in 0.5 mL0.75 % ethamol in balaced salt solution.100 μgVitE group:100 μg a-tocopherol in 0.5 mL0.75 % ethamol in balaced salt solution.The three groups also received 2.5×10 5 fibroblasts injection.On 15 and 30 day after gas/fluid exchange respectively,the sample of vitreous from the three groups and the normal control group were drawn to measure the activity of lipid peroxides(LPO)and superoxide dismutase(SOD).Results:The activity of LPO and SOD were significantly bigger in the model control group than in the normal control group(P<0.01).The activity of LPO was significantly smaller in the two VitE groups than in the model control group(P<0.01).So was SOD(P<0.05).Conclusion:The strong activity of LPO has a close relation with the formation of PVR.A-tocopherol injected intravitreally inhibit the activity of LPO in PVR and show the ability of antioxidation in PVR.