大鼠肾缺血后肾小管上皮及血管内皮细胞超微结构改变

目的：通过电子显微镜观察大鼠。肾小管上皮细胞及肾髓质毛细血管内皮细胞缺血损伤情况。方法：大鼠肾动脉钳夹30、60min,再灌流24h。取肾组织块进行电子显微镜观察。结果：缺血30、60min,肾小管上皮细胞肿胀明显,毛细血管内皮细胞也肿胀,但2组超微结构观察无明显差别；缺血60min再灌流24h,大部分肾小管上皮细胞坏死,毛细血管内皮细胞脱落、肿胀及坏死；缺血30min再灌流24h,部分肾小管上皮细胞坏死或凋亡,毛细血管内皮细胞也有凋亡。结论：缺血损伤的严重程度与细胞死亡方式有关,对电镜观察细胞坏死、凋亡注意点及坏死与凋亡的关系进行了讨论。

Ultrastructure changes of renal proximal tubule epithelial cells and endothelium after ischemia injury in rats

Objective:To study the ischemia damage of renal proximal tubule cells and endothelium by electron mi- croscope.Methods:Renal isehemia was induced by clamping renal arteries for 30 or 60 minutes in rats.The tis- sues were cut into 0.5mm~3 blocks and were fixed for 24 hours after ischemia.The sections were observed under a Hitachi-800 electron microscope.Results:After ischemia,renal proximal tubule cells and endothelium became swollen evidently,and there was no evident ultrastuctural difference between 30 min and 60 min ischemia groups. After 24 h reflow in 60 min ischemia group,many necrosis cells were observed;after 24 h reflow in 30 min isehe- mia group,some necrosis cells and some apoptosis cells were observed.Conclusion:The injury degree of ischemia is related to the cell death pattern.The relationship between necrosis and apoptosis,and the application of elec- tron microscope are discussed.