pEgr-ssEndostatin重组质粒瘤内辐射诱导表达及其抗肿瘤作用

目的 观察pEgr-ssEndostatin基因-放射治疗对移植黑色素瘤B16小鼠的抗肿瘤作用及其在瘤内辐射诱导表达。方法 肿瘤局部注射脂质体包裹的pEgr-ssEndostatin重组质粒后42h，接受20Gyx射线照射，观察放疗后不同时间肿瘤大小，检测放疗后第2天瘤内Endostatin转录水平。结果 pEgr-ssEndostatin基因-放射治疗荷瘤小鼠能够增强放疗疗效；单纯接种pEgr-ssEndostatin质粒组和接种pEgr-ssEndostatin质粒 20Gy照射组小鼠的肿瘤组织内有Endostatin mRNA表达，且pEgr-ssEndostatin质粒照射组的EndostatinmRNA水平高于单纯pEgr-ssEndostatin质粒组。结论 pEgr-Endostatin基因-放射治疗抗肿瘤作用明显优于单纯放疗和单纯基因治疗，肿瘤局部转染重组质粒后照射瘤内Endostatin表达增强。

Expression of pEgr-ssEndostatin in tumor induced by radiation and its anti-tumor effect

Objective To observe the antitumor effect of pEgr|ssEndostatin gene|radiotherapy to murine melanoma and the expression of Endostatin gene in tumor induced by radiation. Methods pEgr|ssEndostatin plasmids were injected locally into the tumor in B16 mice,and 42 h later,the tumors of half of the mice were given 20 Gy X|irradiation.The tumor growth at different times and Endostatin expression 2 days after irradiation were detected. Results Endostatin gene|radiotherapy could enhance the curative effect of radiotherapy.Both pEgr|ssEndostatin group and pEgr|ssEndostatin+20 Gy group had Endostatin mRNA expression,but the mRNA level of pEgr|ssEndostatin+20 Gy group was higher than that of pEgr|ssEndostatin group. Conclusion The antitumor effect of pEgr|ssEndostatin gene|radiotherapy is better than that of radiotherapy and gene therapy only.And Endostatin expression in tumor is enhanced after irradiation.;