郑州地区2型糖尿病肾功能衰竭患者芳香酯酶与基因Q192R多态性的关系

目的 探讨郑州地区汉族人群2型糖尿病慢性肾功能衰竭(DN-CRF)与血清芳香酯酶(ArE／PON1)活性及其192位基因多态性的关系。方法 通过检测2型糖尿病组(DM，121例)、DN-CRF组(123例)、健康对照组(127例)等观察对象的血清ArE／PONl活性及其192位基因多态性、血脂和脂蛋白等，进行分析研究。结果 郑州地区汉族人群中存在有ArE／PON1 192位等位基因Q与R，DN-CRF组Q、R基因频率为0．45和0．55，与DM、对照组比较，差异无统计学意义；两病例组患者血清酶活性均低于对照组，DN-CFR组降低幅度最大；DN-CFR组内RR基因型患者高密度脂蛋白胆固醇(HDL-C)、高密度脂蛋白2胆固醇(HDL2-C)水平低于QQ基因型，总胆固醇(TC)、甘油三酯(TG)和氧化型低密度脂蛋白(oxLDL)高于QQ基因型。结论 DN-CRF组ArE／PONl192位基因多态性与DM、健康对照组间虽差异无统计学意义，但不能排除DM合并DN-CRF与ArE／PON1的192位基因多态性有关；DN-CRF患者血清ArE／PON1活性降低，可能是DM合并DN的危险因素。

Relationship between arylesterase/paraoxonase activity and ArE/PON1 Q192R gene polymorphism and patients with 2 type diabetic renal failure in Zhengzhou population of Han nationality

Objective To explore the relationship between arylesterase/paraoxonase(ArE/PON1) activity and polymorphism of Q192R ArE /PON1 in Chinese Han ethic patients with 2 type diabetes mellitus(type 2 DM) and diabetic nephropathy with renal function failure(DN-CRF). Methods The activity of serum ArE /PON1 and its polymorphism in 192 site, lipid and lipoprotien were determined in patients with diabetes(121 cases), DN-CRF(123 cases), and healthy subjects (127 cases). Results The frequency of the Q allele and R was 0.45 and 0.55 in DN-CRF, which was shown no statistic difference among diabetes and healthy subjects(P> 0.05). But the serum ArE/PON1 activity in diabetic patients with or without CRF was lower than that in healthy subjects. In DN-CRF group, the levels of serum HDL-C and HDL2-C with RR genotype were significantly lower; total cholesterol,triglycerides and oxLDL concentration were higher than those with genotype QQ. Conclusion There were no significant differences on the frequency of the Q and R allele between DN-CRF group and others, but ArE/PON1 gene may be a factor involved in the development of diabetic CRF. ArE/PON1 activity may be a risk factor for DM complicated with DN-CRF.