| 高效液相色谱法测定解热宁颗粒中黄芩苷含量 |
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| 引用本文： | 詹少卿,何振伟,张冬梅,陈桂平.高效液相色谱法测定解热宁颗粒中黄芩苷含量[J].中国现代药物应用,2008,2(21):17-18. |
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| 作者姓名： | 詹少卿 何振伟 张冬梅 陈桂平 |
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| 作者单位： | 詹少卿 (江苏省南通市第一人民医院药剂科,226001)； 何振伟 (江苏省南通市第一人民医院药剂科,226001)； 张冬梅 (江苏省南通市第一人民医院药剂科,226001)； 陈桂平 (江苏省南通市第一人民医院药剂科,226001)； |
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| 摘 要： | 目的测定解热宁颗粒中黄芩苷的含量。方法采用高效液相色谱色谱法,测定条件为：色谱柱为KromasilC18（5μm,4.×250mm）,流动相：甲醇-0.2%磷酸溶液（47：53）;检测波长：276nm;流速：1.0ml/min;柱温：35℃。结果黄芩苷在0.0995-0.7960g范围内线性关系良好,r=0.99994,平均回收率97.40%（RSD=0.31%,n=6）。结论该方法简便、快速、准确,可用于解热宁颗粒中黄芩苷的含量测定。
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| 关 键 词： | 解热宁颗粒 黄芩苷 高效液相色谱 含量测定 |
Determination of Baicalin in Jierening granule by HPLC |
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| Institution: | ZHAN Shao-qing , HE Zhen-wei, Zhang Dong-mei,et al. (Department of Pharmacy, The first People＇s Hospital of Nantong, Nantong 226001, China) |
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| Abstract: | Objective To establish a determination method for baicalin in Jierening capsules. Methods HPLC with Kromasil C18（5μm,4.6 ×250 mm） column was used. The mobile phase was methanol- 0. 2% phosphoric acid （47：53 ）. The detection wavelength was at 276 nm. Results There was good linear relationship between 0. 099 5 - 0. 796 0 g,, r = 0. 999 8. The average recovery was 97.40% and RSD was 0. 81%. Conehmion This method is simple, quick, accurate and provided a scientific basis for the quality controlling of jierening granule. |
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| Keywords: | Jierening granule Baiealin HPLC Quantitative determination |
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