| 短发夹状RNA对GRP78基因在结直肠癌RKO细胞中表达的抑制作用 |
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| 引用本文： | 王丽丽,邢晓明,李玉军.短发夹状RNA对GRP78基因在结直肠癌RKO细胞中表达的抑制作用[J].青岛大学医学院学报,2010,46(5):387-389. |
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| 作者姓名： | 王丽丽 邢晓明 李玉军 |
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| 作者单位： | 青岛大学医学院附属医院病理科,山东,青岛,266003 |
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| 基金项目： | 山东省优秀中青年科学家奖励基金资助项目，山东省自然科学基金资助项目 |
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| 摘 要： | 目的探讨短发夹状RNA对糖调节蛋白78(GRP78)在结直肠癌RKO细胞中表达抑制作用。方法以脂质体Lipofectamine2000介导GRP78短发卡状RNA(shRNA-GRP78)质粒表达载体转染RKO细胞,G418筛选得到稳定表达株,半定量RT-PCR和Westernblooting方法检测GRP78mRNA和蛋白水平变化。结果转染成功并筛选出稳定表达RKO细胞株。与对照组相比,shRNA-GRP78转染RKO细胞GRP78的mRNA及蛋白水平均显著下降(t=7.079、7.510,P<0.05)。结论 shRNA-GRP78能明显抑制RKO细胞GRP78mRNA及蛋白的表达,为进一步在体内外研究GRP78在结直肠癌中的作用奠定了基础。
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| 关 键 词： | 葡萄糖调节蛋白78 结直肠肿瘤 RNA干扰 |
DEPRESSANT EFFECT OF SHORT HAIRPIN RNA ON GRP78 EXPRESSION OF RKO CELLS IN COLORECTAL CARCINOMA |
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| WANG LI-LI,XING XIAO-MING,LI YU-JUN.DEPRESSANT EFFECT OF SHORT HAIRPIN RNA ON GRP78 EXPRESSION OF RKO CELLS IN COLORECTAL CARCINOMA[J].Acta Academiae Medicinae Qingdao Universitatis,2010,46(5):387-389. |
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| Authors: | WANG LI-LI XING XIAO-MING LI YU-JUN |
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| Institution: | WANG LI-LI,XING XIAO-MING,LI YU-JUN(Department of Pathology,Qingdao University Medical College,Qingdao 266003,China) |
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| Abstract: | Objective To explore the supression of glucose-regulated protein 78(GRP78)expression in human colorectal carcinoma cells RKO by short hairpin RNA(shRNA)interference.Methods The shRNA plasmid expression vector(shRNA-GRP78)was transfected into cells with Lipofectamine 2000.After selection with G418,the stable cell clones were attained.GRP78 expression was assayed with real-time quantitative PCR and Western blotting at both mRNA and protein levels.ResultsThe transfection was successful and stable RKO cell clon... |
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| Keywords: | glucose-regulated protein 78 colorectal neoplasms RNA interference |
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