新型胆碱酯酶抑制剂对AD大鼠空间记忆及海马结构胆碱能纤维的影响

目的探讨新型胆碱酯酶抑制剂(NAI)及水迷宫训练对AD大鼠海马结构胆碱能纤维的影响。方法用36只Wistar♂大鼠制作AD动物模型,随机分成3组:NAI组、石杉碱甲组(Hup组)、单纯损伤组(SO组)。水迷宫训练后,采用组织化学方法测定海马结构胆碱能纤维密度。结果①定位航行实验中,NAI组逃避潜伏期较SO组显著缩短(P<0.01)。②空间探索实验中,NAI组跨越各象限平台相应位置次数占总次数百分率较SO组明显增高(P<0.01)。③组化结果显示:NAI组海马结构胆碱能纤维密度较SO组明显增加(P<0.05)。尽管NAI组胆碱能纤维密度高于Hup组,但无统计学意义。结论经NAI治疗及水迷宫行为训练后的AD大鼠,海马结构内胆碱能纤维密度明显增加,提示NAI及水迷宫训练联合作用可促进AD大鼠海马结构胆碱能纤维重建。

Effect of novel AChE inhibitor spatial memory and cholinergic fibers in hippocampal formation of AD rats

Aim To investigate the effects of a novel AChE inhibitor (NAI) and water maze training on cholinergic fiber in hippocampal formation of AD rats. Methods 36 male Wister rats of 3~5 months were chosed to make AD models and randomly divided into 3 groups: NAI-, huptrzin A (Hup)-treatment group and simple-injury group. All groups received Morris water maze training, then were tested the AChE positive fiber density in the hippocampal formation by histochemical method. Results ①During the place navigation test,the NAI-treatment group showed shorter latency than the simple-injury group (P<0.01). ②On spatial probe test, NAI-treatment group showed increased percentage of swim time of crossing the platform compared with that of simple-injury group (P<0.01). ③The histochemical study revealed a higher cholinergic fiber density in hippocampal formation of NAI-treatment group than that of simple-injury group. There was no stactistic difference between NAI- and Hup-treatment groups.Conclusion The results showed that NAI treatment and water maze training can increase the cholinergic fiber density in the hippocampal formation of AD rats indicating the function of NAI treatment and water maze training on cholinergic fiber plasticity in the hippocampal formation of AD rats.