| 反向基因芯片技术检测牙龈卟啉单胞菌致病岛基因 |
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| 引用本文： | 林莉,赵戬,李琛,刘静波,潘亚萍. 反向基因芯片技术检测牙龈卟啉单胞菌致病岛基因[J]. 口腔医学研究, 2010, 26(2): 223-226 |
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| 作者姓名： | 林莉 赵戬 李琛 刘静波 潘亚萍 |
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| 作者单位： | 中国医科大学附属口腔医学院牙周科,辽宁,沈阳,110002 |
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| 基金项目： | 辽宁省教育厅科学技术研究基金资助项目(编号:20061021);;国家自然科学基金资助项目(编号:30772427) |
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| 摘 要： | 目的：应用反向基因芯片技术检测PG0836、PG0838、PG0839基因在慢性牙周炎患者和牙周健康者牙龈卟啉单胞菌（P.gingivalis）中分布,探讨这些基因与牙周临床指数之间的关系。方法：选取41例慢性牙周炎患者,牙周健康者76例,记录临床指标牙周探诊深度、临床附着丧失、探诊出血及牙齿松动度,取龈下菌斑进行细菌分离培养,以临床采集的样本提取的DNA为探针,以抑制消减杂交技术获得的P.gingivalis W83的特异基因片段PG0836、PG0838、PG0839为目标序列,采用Cy5荧光标记目标序列。应用基因芯片技术检测PG0836、PG0838、PG0839基因在病变部位及非病变部位的牙龈卟啉单胞菌中的分布。结果：PG0836、PG0838、PG0839基因在病变部位和非病变部位中的检出率均有统计学差异（P〈0.05）,并且与牙周临床指数PD、CAL、TM相关。结论：PG0836、PG0838、PG0839基因与P.gingivalis的致病性有关。
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| 关 键 词： | 牙龈卟啉单胞菌 致病岛 慢性牙周炎 |
Screening of Pathogenicity Islands Genes of Porphyromonas Gingivalis with Reversing Microarray |
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| LIN Li,ZHAO Jian,LI Chen,et al.. Screening of Pathogenicity Islands Genes of Porphyromonas Gingivalis with Reversing Microarray[J]. Journal of Oral Science Research, 2010, 26(2): 223-226 |
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| Authors: | LIN Li ZHAO Jian LI Chen et al. |
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| Affiliation: | LIN Li,ZHAO Jian,LI Chen,et al.Department of Periodontics,School of Stomatology,China Medical University,Shenyang 110002 |
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| Abstract: | Obiective: To investigate PG0836,PG0838 and PG0839 gene of Porphyromonas gingivalis(P.gingivalis)in subgingival plaque of the chronic periodontitis patients and periodontally healthy subjects,and to find out the relationship between detection rates of PG0836,PG0838,PG0839 and periodontal clinical parameters.Methods: The subjects were 41 Chinese CP patients and 76 periodontally healthy individuals.Clinical parameters were measured and recorded by a single examiner,including bleeding on probing(BOP),clinical ... |
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| Keywords: | Porphyromonas gingivalis Pathogenicity islands Chronic periodontitis |
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