YKL-40表达与子宫内膜癌临床病理因素的关系

目的探讨甲壳质酶蛋白-40（YKL-40）在子宫内膜癌患者的组织和血清中的表达与其临床病理因素的关系。方法采用SP免疫组织化学方法和ELISA方法检测YKL-40在31例子宫内膜癌、10例子宫内膜不典型增生和10例子宫内膜正常者的组织和血清中的表达,并用SPSS 17.0统计软件包分析YKL-40表达与子宫内膜癌的临床病理危险因素的关系。结果①子宫内膜癌患者的YKL-40血清浓度（中位数102.58 ng/ml）明显高于子宫内膜不典型增生（中位数58.20 ng/ml）和正常对照者（中位数37.04 ng/ml）,差异有统计学意义（P〈0.05）;且血清YKL-40表达水平与肿瘤分期呈正相关（P=0.046）,但与组织学分级、淋巴结转移、腹腔冲洗液或腹腔积液细胞学结果无关（P〉0.05）。②YKL-40在子宫内膜癌组织中的阳性表达率为45.2%（14/31）.明显高于子宫内膜不典型增生的10.0%（1/10）和正常对照者的10.0%（1/10）.差异有统计学意义（P〈0.05）,且YKL-40阳性表达与组织学分级呈正相关（P=0.049）,但与临床分期和淋巴结转移无关（P〉0.05）。结论 YKL-40在子宫内膜癌的组织和血清水平均呈高表达,且组织中的阳性表达与肿瘤的组织学分级呈正相关,血清中表达与肿瘤分期呈正相关提示YKL-40可能与子宫内膜癌的发生、发展有关,有望成为子宫内膜癌的诊断和/或预后相关的分子标志物,但尚需进一步验证。

Relations between the expression of YKL-40 and the clinical-histopathologic risk factors of endometrial carcinoma

Objective To explore the expression of YKL-40 protein in tissues and serum of patients with endometrial carcinoma, and to analyze the relations between the expression of YKL-40 protein and clinical-histopathologie risk factors of endometrial carcinoma. Method The expression of YKL-40 protein was detected using immunohistochemistxy （IHC） in endometrial carcinoma tissues from 31 patients, atypical hyperplasia tissues in endometrium from 10 patients and normal endometrial tissues from 10 patients with smooth myoma in uterus, respectively, the concentration of YKL-40 protein in serums from these patients was detected using enzyme linked immunosorbent assay （ELISA）. The SPSS software package 17.0 was applied to analyze the relations between the expression of YKL-40 protein and clinical-histopathologic risk factors of endometrial carcinoma. Result ELISA detection showed that the concentration of YKL-40 protein in serums of patients with endometrial carcinoma （ median = 102. 58 ng/ml） is significantly higher than that of patients with atypical hyperplasiaof endometrium （ median = 58.20 ng/ml） and normal endometrium （ median = 37.04 ng/ml） （P 〈 0. 05） , and the con- centration of YKL-40 protein in serums was increasing with tumor stages, but it was not related to tumor grade, lymphatic metastasis, and cytological outcome of peritoneal washings or ascites （ P 〉 0. 05 ）. IHC detection displayed that the positive expression rate of YKL-40 protein in endometrial cancer tissues is 45.9% （ 14/31 ） , significantly higher than that in atypi- cal hyperplasia of endometrium （ 10. 0% ） and normal endometrimn （ 10. 0% ） （ P 〈 0. 05 ）, and the expression of YKL-40 protein in endometrial cancer tissues was positively correlated with tumor grade （ P 〈 0. 049 ）, but not associated with tumor stages and lymphatic metastasis （ P 〉 0. 05 ）. Conclusion YKL-40 protein was highly expressed in both tissues and serums of patients with endometrial cancer. The YKL-40 protein expression of tissue was related with tumor grade, and the serum level of YKL-40 protein was correlated with tumor stage. Thus YKL-40 protein might be involved in the tumorigene- sis and progression of endometrial carcinoma. Suggesting that YKL-40 protein could be used as a potential tumor marker for diagnosis and prognosis of endometrial carcinoma, furlher investigation of this promising endometrial cancer marker in lar- ger studies is warranted.