游离丙型肝炎病毒核心抗原检测的临床价值探讨

目的:探讨游离丙型肝炎病毒(HCV)核心抗原检测在HCV感染诊断中的价值。方法:采用荧光定量PCR法检测HCV-RNA、ELISA法同步检测抗-HCV和游离HCV核心抗原。结果:191例HCV感染者HCV-RNA的检出率为71·2%(136/191);抗-HCV的检出率为97·4%(186/191);游离HCV核心抗原的检出率为33·0%(63/191)。其中有2例经抗病毒治疗的患者HCV-RNA和抗-HCV检测均阴性,但游离HCV核心抗原检测阳性;另有1例患者抗-HCV阴性,而游离HCV核心抗原阳性,经HCV-RNA证实为HCV感染。27例非HCV感染者HCV-RNA、抗-HCV和游离HCV核心抗原检测结果均为阴性。结论:HCV核心抗原检测作为抗-HCV检验的补充试验对HCV感染的诊断具有重要价值。

Clinical value of the detection of hepatitis C virus core antigen

AIM:To explore the value of detecting hepatitis C virus core antigen in HCV infected diseases.METHODS:The HCV-RNA was tested by fluorescent quantitative polymerase chain reaction(FQ-PCR).The core antigens of HCV and anti-HCV were tested by enzyme-linked immunosorbent assay(ELISA).RESULTS:The positive rate of HCV-RNA in 191 patients was 71.2%,about 136 in 191.The positive rate of anti-HCV was 97.4%,about 186 in 191.The positive rate of HCV core antigen was 33.0%,about 63 in 191.After antiviral therapy for 2 patients,their HCV-RNA and anti-HCV were detected negative but their HCV core antigen was detected positive.In another patient,his anti-HCV was negative but his HCV core antigen was positive.CONCLUSION:As a supplemental test for anti-HCV examination the detection of HCV core antigen is of important clinical value in HCV infection diagnosis.