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| LC-MSn鉴定大鼠体内SIPI的代谢产物 | |
| 引用本文： | 张鹏,仇峰,魏广力,刘昌孝,钟大放.LC-MSn鉴定大鼠体内SIPI的代谢产物[J].中国药学杂志,2007,42(3):219-223. |
| 作者姓名： | 张鹏 仇峰 魏广力 刘昌孝 钟大放 |
| 作者单位： | 1. 沈阳药科大学药物代谢与药物动力学实验室,沈阳,110016 2. 沈阳药科大学药物代谢与药物动力学实验室,沈阳,110016;天津药物研究院,天津药代动力学与药效动力学省部共建国家重点实验室,天津,300193 3. 天津药物研究院,天津药代动力学与药效动力学省部共建国家重点实验室,天津,300193 4. 沈阳药科大学药物代谢与药物动力学实验室,沈阳,110016;中国科学院上海药物研究所,上海,201203 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 目的考察SIPI在大鼠体内的代谢转化。方法采用液相色谱-质谱(LC-MSn)联用技术,检测在单剂量静脉注射给予SIPI后大鼠尿,粪及胆汁中的SIPI及代谢物。色谱柱为Diamonsil C18柱;流动相为甲醇-水-甲酸(40∶60∶0.5),流速为0.5mL·min-1;质谱仪离子源为电喷雾离子源(ESI),正离子方式检测。代谢物经LC-MSn方法分离和分析,通过质谱和色谱行为推测其结构。结果在大鼠尿样中共检测到8种代谢物,在大鼠粪样中共检测到4种代谢物,在大鼠胆汁样品中共检测到3种代谢物。结论SIPI在大鼠体内广泛代谢,形成多种代谢产物。 |
| 关 键 词： | SIPI 液相色谱-质谱 代谢 大鼠 |
| 文章编号： | 1001-2494（2007）03-0219-05 |
| 收稿时间： | 2005-12-24; |
| 修稿时间： | 2005-12-24 |
Characterization of Metabolites of SIPI in Rat by Liquid Chromatography-Mass Spectrometry |
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| ZHANG Peng,QIU Feng,WEI Guang-li,LIU Chang-xiao,ZHONG Da-fang.Characterization of Metabolites of SIPI in Rat by Liquid Chromatography-Mass Spectrometry[J].Chinese Pharmaceutical Journal,2007,42(3):219-223. | |
| Authors: | ZHANG Peng QIU Feng WEI Guang-li LIU Chang-xiao ZHONG Da-fang |
| Institution: | 1. Laboratory of Drug Metabolism and Pharmacokinetics , Shen yang Pharmaceutical University, Shenyang 110016, China; 2. Institute of Meteria Medica , Chinese Academy of Sciences, Shanghai 201203, China; 3. Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjing 300193, China |
| Abstract: | OBJECTIVE To investigate the metabolic profile of SIPI in rats.METHODS Liquid chromatography-tandem mass spectrometry (LC-MSn) was used for the separation and analysis of SIPI and its metabolites in rats urine, feces and bile after an intravenous dose of SIPI. The metabolites were separated using a mobile phase consisting of methanol-water-formic acid (40∶60∶0.5) at 0.5 mL·min-1 with a Diamonsil C18 column. The instrument was operated in positive electrospray ionization (ESI) mode. The metabolites were identified by comparisons of their mass spectra and LC behavior.RESULTS Totally,8 metabolites were detected in rats urine, 4 metabolites were detected in rats feces and 3 metabolites were detected in rats bile.CONCLUSION SIPI underwent extensive metabolic pathways in rats. |
| Keywords: | SIPI |
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