大鼠实验性脑出血后细胞凋亡和神经损伤的关系

目的　研究脑出血后血肿周围水肿带细胞凋亡随出血时相点的变化规律 ,以及脑出血后神经细胞凋亡和神经功能的关系。方法　健康雄性Wistar大鼠体质量 2 0 0～ 2 5 0g ,随机分组 ,脑内尾状核注射分别Ⅳ型胶原酶 肝素生理盐水溶液、单纯生理盐水 ,分为术后 6、12、2 4h、3、7d共 5个时相点。术后在相应时相点评价大鼠神经功能缺损后 ,制作冰冻切片 ,对切片进行HE染色和TUNEL染色 ,之后利用图像分析仪 ,观察阳性细胞数。结果　脑出血后动物出现不同程度神经功能缺损 ,在 3d神经功能损害最严重。出血 6h后血肿周围出现TUNEL阳性细胞 ,3d左右TUNEL阳性细胞范围最广。结论　脑出血后细胞凋亡与神经功能损害程度一致 ,细胞凋亡在脑出血后神经功能损伤中起重要作用。

Relationship between apoptosis and neurological impairment in experimental cerebral hemorrhage in rats

Objective To explore the changes of apoptosis at different time points around the hematoma after cerebral hemorrhage and the relationship between apoptosis and injury of nerve. Methods Healthy male Wistar rats weighing from 200 to 250g were randomly divided into different groups. Collagenase/heparin in saline solution or pure saline solution was injected into the caudate nucleus. Neurological impairment was evaluated at 6, 12, and 24 h, and 3 and 7 d after the injection, respectively. HE and TUNEL stainings were employed for the frozen sections. The positive cells were observed by the image analysis. Results Neurological impairment occurred in each rat after cerebral hemorrhage, and the most severe neurological impairment was found at 3 d after injection. TUNEL positive cells were found at 6 h after injection first, widespread at 3 d after injection. Conclusion The degree of the neurologic impairment is the same as that of the apoptosis. Apoptosis may play an important role in neurological impairment after cerebral hemorrhage.