S-100蛋白、PCNA在胃癌中的表达及意义

目的探讨胃粘膜中S-100阳性细胞(S-100 DC)浸润、增殖细胞核抗原(PCNA)的表达在胃癌发生、发展中的作用及临床意义。方法应用免疫组织化学方法对57例胃癌、18例正常胃粘膜组织中的S-100 DC、PCNA进行检测。结果S-100 DC、PCNA在胃癌组中的表达均显著高于正常胃粘膜组(P<0.05)。在低分化、淋巴结转移阳性和有远处转移胃癌组织中S-100 DC数目明显减少(P<0.01)。PCNA阳性表达率与淋巴结转移、远处转移、分化程度、肿瘤大小密切相关(P<0.05),而与肿瘤浸润深度、临床分期无关(P>0.05)。胃癌组织中S-100 DC与PCNA的表达呈负相关性。结论胃粘膜微环境中S-100 DC与PCNA的变化与胃癌临床病理参数密切相关。胃癌组织内S-100 DC增多可抑制肿瘤细胞的增殖潜力,并具有抗肿瘤转移的作用。

Expression and clinical significance of S - 100 protein and PCNA antigen in gastric carcinoma

Objective To study the clinical significance and effects of the expression of S-100~ dendritic cells(S-100~ DC) and proliferating cell nuclear antigen(PCNA) in the occurrence and development of gastric carcinoma. Methods S-100~ DC and PCNA expression was detected in 43 specimens of gastric carcinoma,24 cases of normal gastric mucosa using immunohistochemistry. Results The expression of S-100~ DC and PCNA in gastric carcinoma was markly higher than in normal gastric mucosa (P<0.05). The number of S-100~ DC in the gastric carcinoma tissues withlow differentiation, lymph node and distant metastasis was significantly lower (P<0.01). The positive rates of PCNA were correlated with lymph node metastasis, diatant metastasis, degree of differentiation, tumor size (P<0.05),and no relation with depth of invasion and clinical stage(P>0.05). A negative relationship was found between S-100~ DC and PCNA expressions in gastric cancer. Conclusion The change of S-100~ DC and HLA-DR in gastric mucosa were closely correlated with the clinical pathological parameters of gastric carcinoma. S-100~ DC in gastric cancer might play a role in inhibiting the growth of tumor cells and anti-tumor metastasis.