| Affiliation: | 1. Division of Infectious Diseases and Immunology, Saitama Children''s Medical Center, Saitama, Japan;2. Division of Pediatric Surgery, Saitama Children''s Medical Center, Saitama, Japan;3. Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan;4. Department of Infectious Diseases, The University of Tokyo Hospital, Tokyo, Japan;5. Department of Clinical Laboratory and Molecular Pathology, Graduate School of Medical and Pharmaceutical Science, University of Toyama, Toyama, Japan |
| Abstract: | A 14-year-old otherwise healthy boy presented with right-sided back pain following high fever. Abdominal computed tomography scan showed a large liver abscess. Klebsiella pneumoniae (KP) was rapidly identified from peripheral blood using the melting temperature mapping (Tm) method, which enables identification of pathogenic microorganisms within four hours after patient sample collection. He was diagnosed with pyogenic liver abscess (PLA) caused by KP on the day of admission. The KP was the hypervirulent (hv) clinical variant (string test positive, serotype K1, sequence type 23, rmpA and magA positive). After intravenous antibiotic therapy and drainage of the abscess, his condition resolved. The highlights of this case report are a healthy child with hypervirulent Klebsiella pneumoniae liver abscess in Japan and the new Tm mapping method for rapid and accurate identification of the pathogenic microorganism. |
