巴豆醛暴露对雄性大鼠的肝损伤作用研究

目的 探讨巴豆醛暴露对雄性大鼠的肝脏损伤。方法 选取SPF级健康雄性Wistar大鼠40只,随机分成高剂量组、中剂量组、低剂量组和对照组4个组,每组10只。巴豆醛经口灌胃分别给予高剂量组8.44 mg/kg、中剂量组4.22 mg/kg、低剂量组2.11 mg/kg,大鼠灌胃量为1 ml/100 g体重。每天灌胃一次,连续染毒28天后处死动物。取外周血进行血生化检验;ELISA法检测血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性;取大鼠主要脏器,称重并计算脏器系数,常规HE染色制作病理标本,观察肝脏组织病理学改变。结果 与对照组相比,高剂量组大鼠体重增重减少,肝脏脏器系数明显增高,且随着染毒剂量的增加,肝脏系数有增高的趋势;血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)水平明显增加,高剂量组和中剂量组的MDA含量升高,SOD含量降低,高剂量组的GSH-PX含量降低,差异均有统计学意义(P< 0.05);与对照组相比,心脏系数、脾脏系数、肾脏系数及血清中总胆红素(TBIL)、尿素(BUN)、尿酸(UA)、肌酐(CRE)、葡萄糖(GLU)、甘油三酯(TG)、总胆固醇(CHOL)水平无明显变化,差异无统计学意义(P> 0.05);病理组织切片显示大鼠肝组织有炎性损伤。结论 巴豆醛暴露对雄性大鼠有肝损伤作用。

Crotonaldehyde Exposure on Male Rat Liver Injury Research

Objective To investigate the crotonaldehyde exposed liver injury in male rats.Methods 40 healthy male Wistar rats were randomly divided into 4 groups:high dose group, middle dose group, low dose group and control group, with 10 rats in each group. Rats in each group were randomly divided into groups. Crotonaldehyde oral gavage were given high dose group 8.44 mg/kg, middle dose group 4.22 mg/kg, low dose group 2.11 mg/kg, rat irrigation gastric volume was 1ml/100g weight. Once every day, the animals were sacrificed after 28 d continuous exposure. Biochemical test was done of the peripheral blood; ELISA method was used for the detection of serum malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH PX) activity; rat organs was weighted and calculated the organ coefficient, the routine hematoxylin and eosin staining produced pathological specimens for observing the liver tissue pathological changes.Results Compared with the control group, high dose group rats body weight decreased, liver index increased significantly, and as the dose increased, the liver coefficient increased; serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels significantly increased whilein high dose group and middle dose group, MDA content increased; the content of SOD decreased; the content of GSH-PX decreased in high dose group, the differences were statistically significant (P< 0.05); compared with the control group, the total bilirubin heart coefficient, speen index, kidney index and serum (TBIL), urea (BUN), uric acid (UA), creatinine (CRE), glucose (GLU), triglyceride (TG), total cholesterol (CHOL) levels had no significant change, the difference was not statistically significant (P> 0.05); the pathological sections showed that liver tissue of rats with inflammatory injury.Conclusion Exposure to crotonaldehyde, liver injury was found in male rats.