Flow Cytometric Analysis of In Vitro Proinflammatory Cytokine Secretion in Peripheral Blood from Multiple Sclerosis Patients |
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| Authors: | Linh T Nguyen Murali Ramanathan Frederick Munschauer Carol Brownscheidle Susan Krantz Margaret Umhauer Colleen Miller Ernesto DeNardin Lawrence D Jacobs |
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| Institution: | (1) Department of Pharmaceutics, State University of New York at Buffalo, Buffalo, New York, 14260;(2) Department of Neurology, Buffalo General Hospital, Buffalo, New York, 14203;(3) Department of Oral Biology and Microbiology, State University of New York at Buffalo, Buffalo, New York, 14260;(4) Department of Pharmaceutics, State University of New York at Buffalo, Buffalo, New York, 14260 |
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| Abstract: | The cytokines, interferon- (IFN-), tumor necrosis factor- (TNF-rpar;, and interleukin-2 (IL-2) are important endogenous proinflammatory proteins and have been linked to disease activity in multiple sclerosis. In this study, we use flow cytometric methodology to compare the secretion of IFN-, IL-2, and TNF- from peripheral blood-derived T cells of multiple sclerosis patients to the secretion in healthy controls. The percentages of IFN-, IL-2, and TNF- secreting cells are not significantly different between multiple sclerosis patients and controls. However, the TNF- secreting CDS cell percentage is correlated with the IFN- and IL-2 secreting CD3 cell percentages in multiple sclerosis patients. In the controls, only the TNF- secreting CD3 cell percentage is correlated with IFN-. These findings show that correlated secretion of cytokines occurs in multiple sclerosis and suggest that concerted intercytokine interactions may play an important role in the disease. |
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| Keywords: | Multiple sclerosis flow cytometry interleukin-2 interferon- " target="_blank">gif" alt="gamma" align="MIDDLE" BORDER="0"> interferon- " target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0"> tumor necrosis factor- " target="_blank">gif" alt="agr" align="BASELINE" BORDER="0"> |
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