丹参多糖的免疫调节活性研究

目的:研究丹参多糖的免疫活性。方法:分别考察丹参多糖对LPS(lipopolysaccharide脂多糖)诱导小鼠脾淋巴细胞增殖反应的影响、对小鼠腹腔巨噬细胞吞噬鸡红细胞的影响、对DNFB(二硝基氟苯)诱发小鼠迟发超敏反应的影响。结论:丹参多糖对小鼠淋巴细胞增值反应有着显著的促进作用;可以显著提高小鼠腹腔巨噬细胞的吞噬作用;可以显著的抑制DNFB所致的小鼠耳廓变应性接触性皮肤炎所致的耳肿胀以及血管通透性的增加,同时能影响主要的免疫器官胸腺、脾脏的脏器指数,显著抑制iNOS、IFN-α及IL-1βmRNA基因表达,具有保护机体免受细胞因子过量表达而受到损伤,显示出较好的免疫调节保护活性。

Study on the Immunocompetence of Polysaccharide Extracted from Root of Salvia miltiorrhiza

Objective:To study the immune activity of polysaccharide extracted from root of Salvia miltiorrhiza.Methods:Investigated the effects of polysaccharide extracted from root of Salvia miltiorrhiza on lymphocyte proliferation response of mouse induced by LPS(the lipopolysaccharide LPS),phagocytosis of the peritoneal macrophage of mice to chick erythrocytes and the mouse models of delayed type hypersensitivity(DTH) response induced by DNFB.Results: Lymphocyte proliferation and phagocytosis of the peritoneal macrophage of mice could be promoted by the polysaccharide,which could inhibit ear edema and capillary permeability increase induced by DNFB and enlarged the thymus and splenic index in mice.The expression of iNOS,IFN-α and IL-1β was inhibited significantly in the treatment group.Conclusion:The polysaccharide extracted from Salvia miltiorrhiza can improve immune function.