| 外源性视黄酸调节乳鼠心肌细胞Tbx1的表达 |
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| 引用本文： | 刘苗,吴小燕,徐佳伟,金润铭. 外源性视黄酸调节乳鼠心肌细胞Tbx1的表达[J]. 临床儿科杂志, 2009, 27(8) |
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| 作者姓名： | 刘苗 吴小燕 徐佳伟 金润铭 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院儿科,湖北武汉,430022 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 研究视黄酸(RA)对心肌细胞Tbx1基因表达的影响.方法 分离培养乳鼠心肌细胞,经不同浓度的RA处理后,采用RT-PCR和Western blot方法分别检测乳鼠心肌细胞Tbx1基因在mRNA和蛋白水平表达的变化.结果 Tbx1基因在乳鼠心肌细胞有少量基础的表达,在予以RA刺激后,1×10-7mol/L RA使细胞内Tbx1基冈mRNA和蛋白含量表达分别增加1.51倍和1.10倍(P均<0.05),1×10-6 mol/L RA使细胞内Tbx1基因mRNA和蛋白含量表达分别增加2.21倍和2.38倍(P均<0.05).结论 RA可以上调乳鼠心肌细胞Tbx1的表达,且此作用呈剂量依赖效应.
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| 关 键 词： | 视黄酸 Tbx1基因 心肌细胞 新生鼠 |
Influence of retinoic acid on the Tbx1 expression in neonatal rat cardiomyocytes |
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| LIU Miao,WU Xiao-yan,XU Jia-wei,JIN Run-ming. Influence of retinoic acid on the Tbx1 expression in neonatal rat cardiomyocytes[J]. The Journal of Clinical Pediatrics, 2009, 27(8) |
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| Authors: | LIU Miao WU Xiao-yan XU Jia-wei JIN Run-ming |
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| Affiliation: | Department of Paediatrics;Wuhan Union Hospital;Huazhong University of Science and Technology;Wuhan 430022;Hubei;China |
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| Abstract: | Objective To study the regulatory mechanism of retinoic acid(RA) on the Tbx1 expression in cardiomyocytes.Methods Ventricular cardiomyocytes were isolated from neonatal rats and cultured,then treated with different concentrations of retinoic acid.The expression level of Tbx1 mRNA and protein were measured by using RT-PCR and Western blot technique respectively.Results Basal expression of Tbx1 in the control group were determined.In comparison with the control group,the expression level of Tbx1 mRNA and prot... |
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| Keywords: | retinoic acid Tbx1 cardiomyocyte neonatal rat |
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