| 13,14双氢15-酮基前列腺素F_(2α)放射免疫测定法和初步应用 |
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| 引用本文： | 程锦轩,史以庆,段金虹,王振纲,陈训华,肖严,李振甲,杨梅芳,马魁榕.13,14双氢15-酮基前列腺素F_(2α)放射免疫测定法和初步应用[J].生殖与避孕,1987(2). |
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| 作者姓名： | 程锦轩 史以庆 段金虹 王振纲 陈训华 肖严 李振甲 杨梅芳 马魁榕 |
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| 作者单位： | 中国医学科学院基础医学研究所药理室，中国医学科学院基础医学研究所药理室，中国医学科学院基础医学研究所药理室，中国医学科学院基础医学研究所药理室，中医研究院骨科研究所，中日友好医院，中国人民解放军301医院，中国人民解放军301医院，中国科学院动物研究所 进修生 |
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| 摘 要： | 采用日本大耳白家免获得了亲和力高,特异性强的13,14-双氢15-酮基前列腺素F_(2α)(PGF_(2α)M)抗血清,建成了灵敏、精确、操作简便的PGF_(2α)M放射免疫测定法(RIA)。测定了人和动物血浆和尿样的正常值,同时观察了醋酸棉酚对大鼠血浆和尿中PGF_(2α)M含量的影响以及妇女月经周期中血浆PGF_(2α)M含量的变化。
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| 关 键 词： | PGF_(2α)M RIA |
RADIOIMMUNOASSAY OF 13,14-DIHYDRO-15-KETO-PROSTAGL-ANDIN F_(2α) (PGF_(2α)M) AND IT'S PRELIMINARY APPLICATION |
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| Cheng Jin-xuan Shi Yi-qing Duan Jin-hong Wang Zhen-gang Chen Xung-hua Xiao Yan.RADIOIMMUNOASSAY OF 13,14-DIHYDRO-15-KETO-PROSTAGL-ANDIN F_(2α) (PGF_(2α)M) AND IT'S PRELIMINARY APPLICATION[J].Reproduction and Contraception,1987(2). |
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| Authors: | Cheng Jin-xuan Shi Yi-qing Duan Jin-hong Wang Zhen-gang Chen Xung-hua Xiao Yan |
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| Abstract: | An antiserum of high affinity and specificity was raised in Japan white rabbit. A sensitive and precise radioimmunoassay (RIA) of 13,14-Dihydro-15-keto prostaglandin F2_α (PGF_(2α) M) was established in our laboratary. The results showed that the mean values((?)±SD) were obtaind in plasma (man 0.41±0.09ng/ml,woman 0.59+0.17ng/ml;rat 0.90±0.40ng/ml; rabbit 2.4±0.14ng/ml; calf 2.12±0.18ng/ml) and in urine (man 11.0±1.3μg/g creatinine, woman 16.2±2.3μg/g creatinine; rat 1.6 0.3μg/g creatinine).At the same time, the effect of gossypol acetate on the amount of PGF_(2α)M in plasma and urine of rat and the change of the amount of PGF_(2α) M in plasma of woman during menstruation were also observed. |
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| Keywords: | PGF_(2α)M RIA |
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