母兔卵巢异体异位移植的实验研究

目的:建立雌兔异体卵巢组织移植模型,探索非血管吻合卵巢组织移植的可行性。方法:以雌兔为实验动物,A组:阳性对照,不做任何处理;B组:阴性对照,切除双侧卵巢;C组:卵巢组织异体异位移植组,分离卵巢组织,切成约1mm×1mm×1mm大的组织块,移植于异体颈部皮下,术后观察阴道脱落细胞涂片变化,同时于移植后第18、25、35、45、55天检测血清E2及P水平,60天处死母兔,对移植物及子宫内膜进行组织学检查。结果:(1)C组3只雌兔阴道呈红色或粉红色、湿润、肿胀,进入发情期;(2)A、C组术后25天血清E2及P水平均高于B组,并逐渐下降,35天达到最低水平,随后又呈上升趋势,至55天已明显高于B组(P<0.05),B组则始终维持在较低水平,未出现升高趋势;(3)A、C组卵巢组织内可见不同发育阶段的卵泡。结论:兔卵巢组织异体异位移植后移植物可存活,并释放内源性激素,对靶器官产生作用。

The experimental study on heterotopic allogenic ovarian transplantation in rabbit

Objective:To assess the ovarian functional recovery after ovarian tissue transplantation without a vascular pedicle in rabbits.Methods:Fourteen young-adult rabbits were divided into four groups:group A,positive control(n=5);group B,negative control(n=4);and group C,heterotopic allogenic ovarian transplantation(n=5).After 18,25,35,45,55 days,the blood samples were collected from their auricle vena.The concentrations of E2 and P were measured by ECL technique.The rabbits were killed on 60 days after the transplantation,and the ovary and uterus were removed,isolated and checked histologically.Results:(1)Three cases in group C reestablished the cycle after heterotopic allogenic transplantation.(2)Compared with group B,serum E2 and P concentrations in group A and C presented statistically significant differences on 25 days after ovarian tissue transplantation(P<0.05).Subsequently,the levels were decreased gradually,and were the lowest on 35 days.Later,serum E2 and P rised renewedly,and statistically significant differences were found on 55 days after transplantation compared to group B(P<0.05).(3)Histology of ovarian tissue and endometrium confirmed the vitality of group A and C.Conclusion:Ovarian tissue transplantation without a vascular pedicle is viable in rabbits,preserving hormonal and fertile functions.