抗原表达异常的急性髓细胞性白血病端粒酶活性的临床分析

目的　观察和探讨抗原表达异常的急性髓细胞性白血病 (AML)患者端粒酶活性及对预后的影响。方法　采用端粒重复序列聚和酶链反应扩增法 (TRAP PCR)检测 30例AML患者骨髓的端粒酶活性。结果　 30例AML患者端粒酶阳性者 1 5例 ,阳性率为 50 %。Ly+ 组 (凡细胞既有髓系抗原表达 ,又有一种或两种以上淋系抗原呈阳性反应 ,阳性率≥ 30 %为Ly+ AML)与Ly- 组 (凡细胞仅表达髓系抗原 ,不伴有淋系抗原表达者为Ly- AML)端粒酶阳性率不一致。Ly+ 组较Ly- 组端粒酶活性增高 (56 %vs 42 % )。结论　端粒酶阳性组与端粒酶阴性组相比 ,在同等化疗强度的基础上 ,端粒酶阳性的AML患者完全缓解率 (CR)明显降低 (33 %vs 73 % ,P <0 0 5) ,表明端粒酶表达异常升高是造血细胞恶性程度高的标志之一 ,检测端粒酶的活性可协助白血病的诊断分型 ,并有助于白血病患者的疗效观察

Clinical analysis on detecting the telomerase activity in acute myeloid leukemia patients with abnormal antigen expression

Objective To further explore th e telomerase activity and its effect on the process and prognosis of acute myeloid leukemia (AML).Methods Thirty patients with AML were subjected to a check of t elomerase activity of bone marrow cells by the polymerase-chain reaction-telom eric repeat amplification protocoal (PCR-TRAP).Results Among 30 AML patients,15 patients′ telomerase was posi tive,with a rate of 50%,in Ly + group and Ly - group the telomerase positive rates were 56% and 42% respectively;the telomerase activity was higher in Ly + group.Complete remission (CR) rates were 33% and 73% in the group with telome rase positive and in the group with telomerase negative respectively ( P <0 05).Conclusion An abnormal higher of telomerase activity may be one of the specific markers for hematopoietic cell′s deteriorative transformation. Checking the telomerase activity may be useful for diagnostic typing of leukemia and observing curative effect.