| 高分辨率熔解曲线分析技术在检测非小细胞肺癌组织EGFR基因突变中的应用 |
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| 引用本文： | 由田,刘政,邱广斌.高分辨率熔解曲线分析技术在检测非小细胞肺癌组织EGFR基因突变中的应用[J].癌变．畸变．突变,2010,22(6):444-447. |
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| 作者姓名： | 由田 刘政 邱广斌 |
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| 作者单位： | 1. 中国医科大学第一临床学院急诊科,辽宁,沈阳,110001;解放军二零二医院检验科,辽宁,沈阳,110003
2. 解放军二零二医院检验科,辽宁,沈阳,110003 |
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| 基金项目： | 辽宁省自然科学基金(20092084) |
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| 摘 要： | 目的：探索高分辨率熔解曲线（high resolution melting,HRM）分析技术在检测肺癌组织表皮生长因子受体（epidermal grouthfactor receptor,EGFR）基因突变中的应用。方法：利用HRM技术,进行人肺癌组织EGFR基因外显子19和21突变筛查,并做测序验证。结果：外显子19的HRM分型结果显示,52例样品中有3种类型熔解曲线,45例样品为A型,5例为B型,2例为C型。外显子21的分析结果显示有2种熔解曲线,49例样品为N型,3例样品为M型。测序结果显示,外显子19 A型熔解曲线代表外显子19的野生型,B型熔解曲线代表杂合性缺失delE746-A750（c.2235-2249del）,C型熔解曲线代表杂合性缺失delL747-S752（c.2240-2257del）。外显子21 N型熔解曲线代表野生型,M型熔解曲线代表杂合性L858R（c.2573T-G）,HRM分析结果与测序结果完全一致。结论：本研究表明HRM分析技术能准确检测EGFR基因突变,是一种适合肺癌组织中EGFR基因突变筛查的方法。
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| 关 键 词： | 非小细胞肺癌 表皮生长因子受体 高分辨率熔解曲线 |
Detecting EGFR mutations in non-small cell lung carcinoma patients using high resolution melting curve analysis |
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| YOU Tian,LIU Zheng,QIU Guang-bin.Detecting EGFR mutations in non-small cell lung carcinoma patients using high resolution melting curve analysis[J].Carcinogenesis,Teratogenesis and Mutagenesis,2010,22(6):444-447. |
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| Authors: | YOU Tian LIU Zheng QIU Guang-bin |
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| Institution: | YOU Tian1,2,LIU Zheng2,QIU Guang-bin2,(1.Department of Emergency Medicine,the First Clinical School of China Medical University,Shenyang 110001,2.Department of Laboratory Medicine,No.202 Hospital of PLA,Shenyang 110003,Liaoning,China) |
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| Abstract: | OBJECTIVE: To evaluate the feasibility and sensitivity of detecting the epidermal growth factor receptor(EGFR) mutation in non-small cell lung carcinoma(NSCLC) patients using high resolution melting(HRM) curve analysis.METHODS: HRM was applied to detect the mutations of EGFR exon 19 and 21 in NSCLC patients,the results were then confirmed by direct sequencing.RESULTS: Three melting curves were obtained by HRM anlysis for EGFR exon 19 and two melting curves for exon 21.Type A(45/52),B(5/52),C(2/52) melting c... |
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| Keywords: | non-small cell lung carcinoma epidermal growth factor receptor high resolution melting curve |
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