益智口服液对阿尔茨海默病大鼠不同脑区细胞凋亡的影响

目的：观察益智口服液对阿尔茨海默病（AD）大鼠不同脑区细胞凋亡的影响。方法：在大鼠CA₁区注入有活性的Aβ₁-40制备AD模型，将30只造模成功的成年雄性清洁级Wistar大鼠随机分为益智口服液组、褪黑素组和AD模型对照组(每组10只)，分别灌胃益智口服液、褪黑素和生理盐水。40 d后处死大鼠，用免疫组化等方法观察细胞凋亡百分率、线粒体膜电位、细胞色素C蛋白质表达变化。结果：与AD模型对照组比较，益智口服液组细胞凋亡百分率明显降低（P<0.05）、线粒体膜电位升高（P<0.05）、细胞色素C蛋白质表达降低（P<0.05）。结论：益智口服液可以通过升高线粒体膜电位、降低细胞色素C蛋白质表达改善AD鼠痴呆状况。

Effects of Yizhikoufuye on apoptosis in different cerebral regions of rats with Alzheimer''''s disease

Objective To observe the effect of YiZhikoufuye(YZ) on apoptosis in the different cerebral regions of experimental Alzheimer's disease(AD) rats.Methods The animal model of AD was set up by stereotaxic damage of CA1 area of rat hippocampus using aggressive Aβ1-40.After operation thirty male Wistar rats were randomly divided into YZ,melatonin and model control groups,10 in each group.The rats were fed with YZ, melatonin and saline,respectively.On the 41th day,the rats were killed.The percentage of apoptotic cells,the changes of mitochondrial membrane potential and the expression level of cytochrome C protein were observed with immunohistochemical methods.Results Compared with control group,YZ could inhibit the apoptotic changes caused by Aβ(P<0.05),inhibit the decrease of mitochondrial membrane potential(P<0.05)and the increase of cytochrome C caused by Aβ.Conclusion YZ has protective function on the Aβ damage by inhibiting the decrease of mitochondrial membrane potential and the increase of cytochrome C.