抗视网膜母细胞瘤细胞株SO-Rb50单克隆抗体相应抗原的提取

目的 从SO-Rb₅₀细胞中提取出抗视网膜母细胞瘤单克隆抗体相应的抗原。 方法 用离子交换层析法从SO-Rb₅₀细胞中初步分离抗原，并利用抗视网膜母细胞瘤单克隆抗体以dot blotting鉴定抗原，然后以十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离纯化出抗原目的蛋白条带，用Westein blotting检测其相对分子质量。 结果 从SO-Rb₅₀细胞裂解液的总蛋白中分离出一条抗原目的蛋白条带，其相对分子质量约为83×10³。 结论 从SO-Rb₅₀细胞裂解液中能够提取出抗视网膜母细胞瘤单克隆抗体对应的抗原。 （中华眼底病杂志，2003，19：152-155）

Separation of SO-Rb50 cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma

bjective To separate the SO-Rb₅₀ cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma. Methods The antigen corresponding to the monoclonal antibody of anti-retinoblastoma was separated elementarily by ion-exchange chromatography, and was identified by dot-blotting using the monoclonal antibody of anti-retinoblastoma. The target protein band of the antigen was separated in light of sodium dodecyl sulfate-polyacrylamide gelelectrophoresis. Results A special unmixed band of SO-Rb₅₀ cells antigen was separated with the relative molecular weight of 83×10³.Conclusion The antigen corresponding to the monoclonal antibody of anti-retinoblastoma could be separated from SO-Rb₅₀cells.(Chin J Ocul Fundus Dis，2003，19：152-155)